ArticleThe Journal of physiology2010
Effects of neuronal nitric oxide synthase inhibition on resting and exercising hindlimb muscle blood flow in the rat.
Article in The Journal of physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
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Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.
- Effects of different dietary supplements on athletic performance in soccer players: a systematic review and network meta-analysis.Journal of the International Society of Sports Nutrition · 2025Pooled it
- Acute effects of citrulline malate and L-arginine, alone and in combination, on anaerobic performance indicators in highly trained taekwondo athletes.Frontiers in nutrition · 2026Article
- What Is the Best Experimental Model for Developing Novel Therapeutics in Peripheral Artery Disease?Arteriosclerosis, thrombosis, and vascular biology · 2024Review
- Does sympathetic vasoconstriction contribute to metabolism: Perfusion matching in exercising skeletal muscle?Frontiers in physiology · 2022Review
- Nitric oxide synthase inhibition with N(G)-monomethyl-l-arginine: Determining the window of effect in the human vasculature.Nitric oxide : biology and chemistry · 2020Article
- Rapid changes in the microvascular circulation of skeletal muscle impair insulin delivery during sepsis.American journal of physiology. Endocrinology and metabolism · 2019Article
- Acute Nitric Oxide Synthase Inhibition Accelerates Transendothelial Insulin Efflux In Vivo.Diabetes · 2018Article
- Neuronal nitric oxide synthase regulation of skeletal muscle functional hyperemia: exercise training and moderate compensated heart failure.Nitric oxide : biology and chemistry · 2018Article
- The effect of nitric oxide synthase inhibition with and without inhibition of prostaglandins on blood flow in different human skeletal muscles.European journal of applied physiology · 2017Article
- Effect of sodium nitrite on local control of contracting skeletal muscle microvascular oxygen pressure in healthy rats.Journal of applied physiology (Bethesda, Md. : 1985) · 2017Article
- Heterogeneity of Muscle Blood Flow and Metabolism: Influence of Exercise, Aging, and Disease States.Exercise and sport sciences reviews · 2015Review
- Imaging of small animal peripheral artery disease models: recent advancements and translational potential.International journal of molecular sciences · 2015Review
- Regulation of increased blood flow (hyperemia) to muscles during exercise: a hierarchy of competing physiological needs.Physiological reviews · 2015Review
- Exercise training augments neuronal nitric oxide synthase-mediated inhibition of sympathetic vasoconstriction in contracting skeletal muscle of rats.The Journal of physiology · 2014Article
- Muscle fibre-type dependence of neuronal nitric oxide synthase-mediated vascular control in the rat during high speed treadmill running.The Journal of physiology · 2013Article
- Neuronal nitric oxide synthase inhibition and regional sympathetic nerve discharge: implications for peripheral vascular control.Respiratory physiology & neurobiology · 2013Article
- Sympathetic activation increases NO release from eNOS but neither eNOS nor nNOS play an essential role in exercise hyperemia in the human forearm.American journal of physiology. Heart and circulatory physiology · 2013Article
- Neuronal nitric oxide synthase is indispensable for the cardiac adaptive effects of exercise.Basic research in cardiology · 2013Article
- Impact of dietary nitrate supplementation via beetroot juice on exercising muscle vascular control in rats.The Journal of physiology · 2013Article
- Effects of neuronal nitric oxide synthase inhibition on microvascular and contractile function in skeletal muscle of aged rats.American journal of physiology. Heart and circulatory physiology · 2012Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nitric oxide (NO) derived from endothelial NO synthase (eNOS) is an integral mediator of vascular control during muscle contractions. However, it is not known whether neuronal NOS (nNOS)-derived NO regulates tissue hyperaemia in healthy subjects, particularly during exercise. We tested the hypothesis that selective nNOS inhibition would reduce blood flow and vascular conductance (VC) in rat hindlimb locomotor muscle(s), kidneys and splanchnic organs at rest and during dynamic treadmill exercise (20 m min(-1), 10% grade). Nineteen male Sprague-Dawley rats (555 +/- 23 g) were assigned to either rest (n = 9) or exercise (n = 10) groups. Blood flow and VC were determined via radiolabelled microspheres before and after the intra-arterial administration of the selective nNOS inhibitor S-methyl-L-thiocitrulline (SMTC, 2.1 +/- 0.1 micromol kg(-1)). Total hindlimb muscle blood flow (control: 20 +/- 2 ml min(-1) 100g(-1), SMTC: 12 +/- 2 ml min(-1) 100g(-1), P < 0.05) and VC (control: 0.16 +/- 0.02 ml min(-1) 100 g(-1) mmHg(1), SMTC: 0.09 +/- 0.01 ml min(-1) 100 g(-1) mmHg(-1), P < 0.05) were reduced substantially at rest. Moreover, the magnitude of the absolute reduction in blood flow and VC correlated (P < 0.05) with the proportion of oxidative muscle fibres found in the individual muscles or muscle parts of the hindlimb. During exercise, total hindlimb blood flow (control: 108 +/- 7 ml min(-1) 100 g(-1), SMTC: 105 +/- 8 ml min(-1) 100 g(-1)) and VC (control: 0.77 +/- 0.06 ml min(-1) 100g(-1) mmHg(-1); SMTC: 0.70 +/- 0.05 ml min(-1) 100g(-1) mmHg(-1)) were not different (P > 0.05) between control and SMTC conditions. SMTC reduced (P < 0.05) blood flow and VC at rest and during exercise in the kidneys, adrenals and liver. These results enhance our understanding of the role of NO-mediated circulatory control by demonstrating that nNOS does not appear to subserve an obligatory role in the exercising muscle hyperaemic response in the rat.
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