ArticleNucleic acids research2010
A microfluidic oligonucleotide synthesizer.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed.
- Ligation-induced DNA self-assembly.Nucleic acids research · 2025Article
- DNA storage: The future direction for medical cold data storage.Synthetic and systems biotechnology · 2025Review
- Droplet-based μChopper device with a 3D-printed pneumatic valving layer and a simple photometer for absorbance based fructosamine quantification in human serum.The Analyst · 2023Article
- Uncertainties in synthetic DNA-based data storage.Nucleic acids research · 2021Review
- Monodisperse drops templated by 3D-structured microparticles.Science advances · 2020Article
- 3DμF - Interactive Design Environment for Continuous Flow Microfluidic Devices.Scientific reports · 2019Article
- An oligonucleotide synthesizer based on a microreactor chip and an inkjet printer.Scientific reports · 2019Article
- A microfluidic chip with a staircase pH gradient generator, a packed column and a fraction collector for chromatofocusing of proteins.Electrophoresis · 2018Article
- Review
- Open-source, community-driven microfluidics with Metafluidics.Nature biotechnology · 2017Article
- Next generation gene synthesis: From microarrays to genomes.Engineering in life sciences · 2017Review
- Nucleic acid memory.Nature materials · 2016Article
- Article
- DNA-library assembly programmed by on-demand nano-liter droplets from a custom microfluidic chip.Biomicrofluidics · 2015Article
- Engineering protocells: prospects for self-assembly and nanoscale production-lines.Life (Basel, Switzerland) · 2015Review
- Next generation 1536-well oligonucleotide synthesizer with on-the-fly dispense.Journal of biotechnology · 2014Article
- New tools and new biology: recent miniaturized systems for molecular and cellular biology.Molecules and cells · 2013Review
- Frontiers and approaches to chemical synthesis of oligodeoxyribonucleotides.Molecules (Basel, Switzerland) · 2013Review
- Microfluidic Paper-Based Analytical Devices (μPADs) and Micro Total Analysis Systems (μTAS): Development, Applications and Future Trends.Chromatographia · 2013Article
- Revisiting lab-on-a-chip technology for drug discovery.Nature reviews. Drug discovery · 2012Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
De novo gene and genome synthesis enables the design of any sequence without the requirement of a pre-existing template as in traditional genetic engineering methods. The ability to mass produce synthetic genes holds great potential for biological research, but widespread availability of de novo DNA constructs is currently hampered by their high cost. In this work, we describe a microfluidic platform for parallel solid phase synthesis of oligonucleotides that can greatly reduce the cost of gene synthesis by reducing reagent consumption (by 100-fold) while maintaining a approximately 100 pmol synthesis scale so there is no need for amplification before assembly. Sixteen oligonucleotides were synthesized in parallel on this platform and then successfully used in a ligation-mediated assembly method to generate DNA constructs approximately 200 bp in length.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.