ArticleBiochemical and biophysical research communications2010
A survey of carbonic anhydrase mRNA expression in enamel cells.
Article in Biochemical and biophysical research communications, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 35 citations in OpenAlex.
- Enamel matrix acidification caused by loss of amelogenin phosphorylation disrupts ameloblast pH-regulatory machinery.JBMR plus · 2026Article
- Review
- Role of amelogenin phosphorylation in regulating dental enamel formation.Matrix biology : journal of the International Society for Matrix Biology · 2024Article
- Identification of stages of amelogenesis in the continuously growing mandiblular incisor of C57BL/6J male mice throughout life using molar teeth as landmarks.Frontiers in physiology · 2023Article
- Genetic variants in taste genes play a role in oral microbial composition and severe early childhood caries.iScience · 2022Article
- Loss of biological control of enamel mineralization in amelogenin-phosphorylation-deficient mice.Journal of structural biology · 2022Article
- New Histamine-Related Five-Membered N-Heterocycle Derivatives as Carbonic Anhydrase I Activators.Molecules (Basel, Switzerland) · 2022Article
- G protein-coupled receptorThe Journal of biological chemistry · 2020Article
- Exploration of the residues modulating the catalytic features of human carbonic anhydrase XIII by a site-specific mutagenesis approach.Journal of enzyme inhibition and medicinal chemistry · 2019Article
- Bicarbonate Transport During Enamel Maturation.Calcified tissue international · 2017Review
- Enamel: Molecular identity of its transepithelial ion transport system.Cell calcium · 2017Review
- DENTAL ENAMEL FORMATION AND IMPLICATIONS FOR ORAL HEALTH AND DISEASE.Physiological reviews · 2017Review
- DLX3-Dependent Regulation of Ion Transporters and Carbonic Anhydrases is Crucial for Enamel Mineralization.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2017Article
- Evidence for Bicarbonate Secretion by Ameloblasts in a Novel Cellular Model.Journal of dental research · 2016Article
- Interactions of amelogenin with phospholipids.Biopolymers · 2015Article
- Article
- Expression patterns and subcellular localization of carbonic anhydrases are developmentally regulated during tooth formation.PloS one · 2014Article
- Protein-mediated enamel mineralization.Frontiers in bioscience (Landmark edition) · 2012Review
- Requirements for ion and solute transport, and pH regulation during enamel maturation.Journal of cellular physiology · 2012Article
- Expression of the sodium/calcium/potassium exchanger, NCKX4, in ameloblasts.Cells, tissues, organs · 2012Article
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
Abstract
Enamel formation requires rigid control of pH homeostasis during all stages of development to prevent disruptions to crystal growth. The acceleration of the generation of bicarbonate by carbonic anhydrases (CA) has been suggested as one of the pathways used by ameloblasts cells to regulate extracellular pH yet only two isozymes (CA II and CA VI) have been reported to date during enamel formation. The mammalian CA family contains 16 different isoforms of which 13 are enzymatically active. We have conducted a systematic screening by RT-PCR on the expression of all known CA isoforms in mouse enamel organ epithelium (EOE) cells dissected from new born, in secretory ameloblasts derived from 7-day-old animals, and in the LS8 ameloblast cell line. Results show that all CA isoforms are expressed by EOE/ameloblast cells in vivo. The most highly expressed are the catalytic isozymes CA II, VI, IX, and XIII, and the acatalytic CA XI isoform. Only minor differences were found in CA expression levels between 1-day EOE cells and 7-day-old secretory-stage ameloblasts, whereas LS8 cells expressed fewer CA isoforms than both of these. The broad expression of CAs by ameloblasts reported here contributes to our understanding of pH homeostasis during enamel development and demonstrates its complexity. Our results also highlight the critical role that regulation of pH plays during the development of enamel.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.