ArticleBMC plant biology2010
STIL, a peculiar molecule from styles, specifically dephosphorylates the pollen receptor kinase LePRK2 and stimulates pollen tube growth in vitro.
Article in BMC plant biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 34 citations in OpenAlex.
- Transcriptome and Metabolome Analyses Reveal a Complex Stigma Microenvironment for Pollen Tube Growth in Tobacco.International journal of molecular sciences · 2024Article
- Kinase Partner Protein Plays a Key Role in Controlling the Speed and Shape of Pollen Tube Growth in Tomato.Plant physiology · 2020Article
- The Long Journey of Pollen Tube in the Pistil.International journal of molecular sciences · 2018Review
- Phylogenetic analysis of STK gene family and Usp domain in maize.Molecular biology reports · 2014Article
- Overexpression of the tomato pollen receptor kinase LePRK1 rewires pollen tube growth to a blebbing mode.The Plant cell · 2014Article
- Tomato Pistil Factor STIG1 Promotes in Vivo Pollen Tube Growth by Binding to Phosphatidylinositol 3-Phosphate and the Extracellular Domain of the Pollen Receptor Kinase LePRK2.The Plant cell · 2014Article
- Signaling in pollen tube growth: crosstalk, feedback, and missing links.Molecular plant · 2013Review
- Sulfinylated azadecalins act as functional mimics of a pollen germination stimulant in Arabidopsis pistils.The Plant journal : for cell and molecular biology · 2011Article
- Whole genome analysis of gene expression reveals coordinated activation of signaling and metabolic pathways during pollen-pistil interactions in Arabidopsis.Plant physiology · 2011Article
- Mutations in two putative phosphorylation motifs in the tomato pollen receptor kinase LePRK2 show antagonistic effects on pollen tube length.The Journal of biological chemistry · 2011Article
- Receptor-like kinases as surface regulators for RAC/ROP-mediated pollen tube growth and interaction with the pistil.AoB PLANTS · 2011Article
- FERONIA receptor-like kinase regulates RHO GTPase signaling of root hair development.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundLePRK1 and LePRK2 are two pollen receptor kinases localized to the plasma membrane, where they are present in a high molecular weight complex (LePRK complex). LePRK2 is phosphorylated in mature and germinated pollen, but is dephosphorylated when pollen membranes are incubated with tomato or tobacco style extracts.
resultsHere we show that LePRK2 dephosphorylation is mediated by a heat-, acid-, base-, DTT- and protease-resistant component from tobacco styles. Using LePRK2 phosphorylation as a tracking assay for purification, style exudates were subjected to chloroform extraction, anionic exchange, and C18 reverse-phase chromatography columns. We finally obtained a single ~3,550 Da compound (as determined by UV-MALDI-TOF MS) that we named STIL (for Style Interactor for LePRKs). STIL increased pollen tube lengths of in vitro germinated pollen in a dose-dependent manner.
conclusionWe propose that the LePRK complex perceives STIL, resulting in LePRK2 dephosphorylation and an increase in pollen tube growth.
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