ArticleBMC genomics2010
Comparative gene expression profiling between human cultured myotubes and skeletal muscle tissue.
Article in BMC genomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 40 citations in OpenAlex.
- Global mRNA sequencing of human skeletal muscle: Search for novel exercise-regulated myokines.Molecular metabolism · 2017Trial
- Delineating transcriptomic signatures of in vitro human skeletal muscle models in comparison to in vivo references.Stem cell reports · 2025Article
- Altered Transcriptome Signature in Primary Human Myotubes Exposed to Inclusion Body Myositis Serum: A Pilot Case Comparison of Anti-cN1A Positive and Negative Sera.Muscles (Basel, Switzerland) · 2025Article
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- Genome-wide investigations reveal the population structure and selection signatures of Nigerian cattle adaptation in the sub-Saharan tropics.BMC genomics · 2022Article
- Non-cell autonomous mechanisms control mitochondrial gene dysregulation in polycystic ovary syndrome.Journal of molecular endocrinology · 2021Article
- Comparative Transcriptome Profile Analysis ofFrontiers in genetics · 2020Article
- Adipose tissue and serum CCDC80 in obesity and its association with related metabolic disease.Molecular medicine (Cambridge, Mass.) · 2017Article
- Nonlinear transcriptomic response to dietary fat intake in the small intestine of C57BL/6J mice.BMC genomics · 2016Article
- Establishment of a new quality control and vaccine safety test for influenza vaccines and adjuvants using gene expression profiling.PloS one · 2015Article
- Genome-wide association study identified copy number variants important for appendicular lean mass.PloS one · 2014Article
- Expressed sequence tags for bovine muscle satellite cells, myotube formed-cells and adipocyte-like cells.PloS one · 2013Article
- Early insights into the function of KIAA1199, a markedly overexpressed protein in human colorectal tumors.PloS one · 2013Article
- Article
- Differential pattern of glycogen accumulation after protein phosphatase 1 glycogen-targeting subunit PPP1R6 overexpression, compared to PPP1R3C and PPP1R3A, in skeletal muscle cells.BMC biochemistry · 2011Article
- Gene expression during normal and FSHD myogenesis.BMC medical genomics · 2011Article
- A versatile single-plasmid system for tissue-specific and inducible control of gene expression in transgenic mice.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2011Article
- Microgenomic analysis in skeletal muscle: expression signatures of individual fast and slow myofibers.PloS one · 2011Article
- Gene expression profiling of skeletal muscle of nursing piglets.International journal of biological sciences · 2010Article
- Article
Corrections and comments
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundA high-sensitivity DNA microarray platform requiring nanograms of RNA input facilitates the application of transcriptome analysis to individual skeletal muscle (SM) tissue samples. Culturing myotubes from SM-biopsies enables investigating transcriptional defects and assaying therapeutic strategies. This study compares the transcriptome of aneurally cultured human SM cells versus that of tissue biopsies.
resultsWe used the Illumina expression BeadChips to determine the transcriptomic differences between tissue and cultured SM samples from five individuals. Changes in the expression of several genes were confirmed by QuantiGene Plex assay or reverse transcription real-time PCR. In cultured myotubes compared to the tissue, 1216 genes were regulated: 583 down and 633 up. Gene ontology analysis showed that downregulated genes were mainly associated with cytoplasm, particularly mitochondria, and involved in metabolism and the muscle-system/contraction process. Upregulated genes were predominantly related to cytoplasm, endoplasmic reticulum, and extracellular matrix. The most significantly regulated pathway was mitochondrial dysfunction. Apoptosis genes were also modulated. Among the most downregulated genes detected in this study were genes encoding metabolic proteins AMPD1, PYGM, CPT1B and UCP3, muscle-system proteins TMOD4, MYBPC1, MYOZ1 and XIRP2, the proteolytic CAPN3 and the myogenic regulator MYF6. Coordinated reduced expression of five members of the GIMAP gene family, which form a cluster on chromosome 7, was shown, and the GIMAP4-reduction was validated. Within the most upregulated group were genes encoding senescence/apoptosis-related proteins CDKN1A and KIAA1199 and potential regulatory factors HIF1A, TOP2A and CCDC80.
conclusionsCultured muscle cells display reductive metabolic and muscle-system transcriptome adaptations as observed in muscle atrophy and they activate tissue-remodeling and senescence/apoptosis processes.
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