ArticleProtoplasma2010
Simulated microgravity perturbs actin polymerization to promote nitric oxide-associated migration in human immortalized Eahy926 cells.
Article in Protoplasma, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 50 citations in OpenAlex.
- Impact of Microgravity on Cytoskeletal Dynamics, Protein Transport, and Signaling Networks: Potential Therapeutic Opportunities for Skin Health.International journal of molecular sciences · 2026Review
- Effects of altered gravity on adrenergic-mediated cAMP signalling in intact cells.NPJ microgravity · 2026Article
- Aging and Altered Gravity: A Cellular Perspective.FASEB journal : official publication of the Federation of American Societies for Experimental Biology · 2025Review
- Modelling large scale artery haemodynamics from the heart to the eye in response to simulated microgravity.NPJ microgravity · 2024Article
- Complement factor H inhibits endothelial cell migration through suppression of STAT3 signaling.Experimental and therapeutic medicine · 2023Article
- Cardiovascular, Lymphatic, and Ocular Health in Space.Life (Basel, Switzerland) · 2022Review
- Effect of Microgravity on Endothelial Cell Function, Angiogenesis, and Vessel Remodeling During Wound Healing.Frontiers in bioengineering and biotechnology · 2021Review
- Spaceflight Induced Disorders: Potential Nutritional Countermeasures.Frontiers in bioengineering and biotechnology · 2021Review
- Interactome of miRNAs and transcriptome of human umbilical cord endothelial cells exposed to short-term simulated microgravity.NPJ microgravity · 2020Article
- The subsequent biological effects of simulated microgravity on endothelial cell growth in HUVECs.Chinese journal of traumatology = Zhonghua chuang shang za zhi · 2018Article
- Clinorotation-induced autophagy via HDM2-p53-mTOR pathway enhances cell migration in vascular endothelial cells.Cell death & disease · 2018Article
- Microgravity-Induced Alterations of Inflammation-Related Mechanotransduction in Endothelial Cells on Board SJ-10 Satellite.Frontiers in physiology · 2018Article
- PTEN/FOXO3/AKT pathway regulates cell death and mediates morphogenetic differentiation of Colorectal Cancer Cells under Simulated Microgravity.Scientific reports · 2017Article
- Fluid Dynamics Appearing during Simulated Microgravity Using Random Positioning Machines.PloS one · 2017Article
- Simulated microgravity inhibits the migration of mesenchymal stem cells by remodeling actin cytoskeleton and increasing cell stiffness.Cytotechnology · 2016Article
- Mechanical perturbations trigger endothelial nitric oxide synthase activity in human red blood cells.Scientific reports · 2016Article
- The impact of microgravity and hypergravity on endothelial cells.BioMed research international · 2015Review
- Growing tissues in real and simulated microgravity: new methods for tissue engineering.Tissue engineering. Part B, Reviews · 2014Review
- Expression of inducible nitric oxide synthase (iNOS) in microglia of the developing quail retina.PloS one · 2014Article
- The impact of simulated and real microgravity on bone cells and mesenchymal stem cells.BioMed research international · 2014Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Microgravity causes endothelium dysfunctions and vascular endothelium remodeling in astronauts returning from space flight. Cardiovascular deconditioning occurs as a consequence of an adaptive response to microgravity partially due to the effects exerted at cellular level. Directional migration of endothelial cell which are central in maintaining the structural integrity of vascular walls is regulated by chemotactic, haptotactic, and mechanotactic stimuli which are essential for vasculogenesis. We explored the migration property of transformed endothelial cells (EC) exposed to 2-h microgravity, simulated using a three-dimensional clinostat constructed based on blueprint published by the Fokker Space, Netherlands. Migration of EC was measured using the scrap wound healing in the presence or absence of actin polymerization inhibitor-cytochalasin D (CD) in Eahy926 cell lines. Simulated microgravity increased cellular migration by 25% while CD-blocked microgravity induced cellular migration. The key migratory structures of cells, filopodia and lamellipodia, formed by EC were more in simulated microgravity compared to gravity. Parallel experiments with phalloidin and diaminorhodamine-4M (DAR-4M) showed that simulated microgravity caused actin rearrangements that lead to 25% increase in nitric oxide production. Further nitric oxide measurements showed a higher nitric oxide production which was not abrogated by phosphoinositol 3 kinase inhibitor (Wortmanin). Bradykinin, an inducer of nitric oxide, prompted two folds higher nitric oxide production along with simulated microgravity in a synergistic manner. We suggest that limited exposure to simulated microgravity increases Eahy926 cell migration by modulating actin and releasing nitric oxide.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.