ArticlePloS one2010
Xenopus meiotic microtubule-associated interactome.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
23 citing papers in PubMed, 45 citations in OpenAlex.
- Article
- Lithium-induced neuroprotection in bipolar disorder: Translational insights into cytoskeletal and proteomic mechanisms.IBRO neuroscience reports · 2025Review
- Interplay between microtubule interactome, myonuclei mechanotransduction, and positioning in myopathies.Nucleus (Austin, Tex.) · 2025Review
- Microtubule association of TRIM3 revealed by differential extraction proteomics.Journal of cell science · 2024Article
- The Joubert syndrome protein CEP41 is excluded from the distal segment of cilia inmicroPublication biology · 2021Article
- Echinoderm Microtubule Associated Protein Like 1 Is Indispensable for Oocyte Spindle Assembly and Meiotic Progression in Mice.Frontiers in cell and developmental biology · 2021Article
- Oocyte competence is maintained by mCell death and differentiation · 2020Article
- Family-based exome sequencing and case-control analysis implicate CEP41 as an ASD gene.Translational psychiatry · 2019Article
- Proteomic Profiling of Microtubule Self-organization in M-phase.Molecular & cellular proteomics : MCP · 2018Article
- Quantitative Proteomics of Xenopus Embryos I, Sample Preparation.Methods in molecular biology (Clifton, N.J.) · 2018Article
- Proteomic Analysis of Microtubule Interacting Proteins over the Course of Xylem Tracheary Element Formation in Arabidopsis.The Plant cell · 2015Article
- Structural maintenance of chromosome (SMC) proteins link microtubule stability to genome integrity.The Journal of biological chemistry · 2014Article
- A microtubule-associated zinc finger protein, BuGZ, regulates mitotic chromosome alignment by ensuring Bub3 stability and kinetochore targeting.Developmental cell · 2014Article
- Purification and characterization of novel microtubule-associated proteins from Arabidopsis cell suspension cultures.Plant physiology · 2013Article
- Cell cycle regulation of microtubule interactomes: multi-layered regulation is critical for the interphase/mitosis transition.Molecular & cellular proteomics : MCP · 2013Article
- Plectin-intermediate filament partnership in skin, skeletal muscle, and peripheral nerve.Histochemistry and cell biology · 2013Review
- Production of Xenopus tropicalis egg extracts to identify microtubule-associated RNAs.Journal of visualized experiments : JoVE · 2013Article
- Intermediate filament-associated cytolinker plectin 1c destabilizes microtubules in keratinocytes.Molecular biology of the cell · 2013Article
- A near-infrared fluorophore for live-cell super-resolution microscopy of cellular proteins.Nature chemistry · 2013Article
- Arena3D: visualizing time-driven phenotypic differences in biological systems.BMC bioinformatics · 2012Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
In metazoan oocytes the assembly of a microtubule-based spindle depends on the activity of a large number of accessory non-tubulin proteins, many of which remain unknown. In this work we isolated the microtubule-bound proteins from Xenopus eggs. Using mass spectrometry we identified 318 proteins, only 43 of which are known to bind microtubules. To integrate our results, we compiled for the first time a network of the meiotic microtubule-related interactome. The map reveals numerous interactions between spindle microtubules and the newly identified non-tubulin spindle components and highlights proteins absent from the mitotic spindle proteome. To validate newly identified spindle components, we expressed as GFP-fusions nine proteins identified by us and for first time demonstrated that Mgc68500, Loc398535, Nif3l1bp1/THOC7, LSM14A/RAP55A, TSGA14/CEP41, Mgc80361 and Mgc81475 are associated with spindles in egg extracts or in somatic cells. Furthermore, we showed that transfection of HeLa cells with siRNAs, corresponding to the human orthologue of Mgc81475 dramatically perturbs spindle formation in HeLa cells. These results show that our approach to the identification of the Xenopus microtubule-associated proteome yielded bona fide factors with a role in spindle assembly.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.