ArticlePloS one2010
The Nanos3-3'UTR is required for germ cell specific NANOS3 expression in mouse embryos.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 22 citations in OpenAlex.
- Clonal analysis reveals differential PGC contributions to the early germline and ovarian reserve.bioRxiv : the preprint server for biology · 2025Article
- Both 20S and 19S proteasome components are essential for meiosis in male mice.Zoological research · 2025Article
- Animal models for COVID-19: advances, gaps and perspectives.Signal transduction and targeted therapy · 2022Review
- Sexual determination in zebrafish.Cellular and molecular life sciences : CMLS · 2021Review
- Zebrafish dazl regulates cystogenesis and germline stem cell specification during the primordial germ cell to germline stem cell transition.Development (Cambridge, England) · 2021Article
- Germ cell differentiation requires Tdrd7-dependent chromatin and transcriptome reprogramming marked by germ plasm relocalization.Developmental cell · 2021Article
- Visualization of Turbot (Scophthalmus maximus) Primordial Germ Cells in vivo Using Fluorescent Protein Mediated by the 3' Untranslated Region of nanos3 or vasa Gene.Marine biotechnology (New York, N.Y.) · 2019Article
- Differential Nanos 2 protein stability results in selective germ cell accumulation in the sea urchin.Developmental biology · 2016Article
- Germ Line Versus Soma in the Transition from Egg to Embryo.Current topics in developmental biology · 2015Review
- Conserved mechanisms for germ cell-specific localization of nanos3 transcripts in teleost species with aquaculture significance.Marine biotechnology (New York, N.Y.) · 2014Article
- The 3'UTR of nanos2 directs enrichment in the germ cell lineage of the sea urchin.Developmental biology · 2013Article
- An E-cadherin-mediated hitchhiking mechanism for C. elegans germ cell internalization during gastrulation.Development (Cambridge, England) · 2012Article
- Molecular regulation of the mitosis/meiosis decision in multicellular organisms.Cold Spring Harbor perspectives in biology · 2011Review
Corrections and comments
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Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe regulation of gene expression via a 3' untranslated region (UTR) plays essential roles in the discrimination of the germ cell lineage from somatic cells during embryogenesis. This is fundamental to the continuation of a species. Mouse NANOS3 is an essential protein required for the germ cell maintenance and is specifically expressed in these cells. However, the regulatory mechanisms that restrict the expression of this gene in the germ cells is largely unknown at present. METHODOLOGY/PRINCIPAL
findingsIn our current study, we show that differences in the stability of Nanos3 mRNA between germ cells and somatic cells is brought about in a 3'UTR-dependent manner in mouse embryos. Although Nanos3 is transcribed in both cell lineages, it is efficiently translated only in the germ lineage. We also find that the translational suppression of NANOS3 in somatic cells is caused by a 3'UTR-mediated mRNA destabilizing mechanism. Surprisingly, even when under the control of the CAG promoter which induces strong ubiquitous transcription in both germ cells and somatic cells, the addition of the Nanos3-3'UTR sequence to the coding region of exogenous gene was effective in restricting protein expression in germ cells. CONCLUSIONS/SIGNIFICANCE: Our current study thus suggests that Nanos3-3'UTR has an essential role in translational control in the mouse embryo.
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