ArticleActa pharmacologica Sinica2010
A mutant of HBx (HBxDelta127) promotes hepatoma cell growth via sterol regulatory element binding protein 1c involving 5-lipoxygenase.
Article in Acta pharmacologica Sinica, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- Arachidonic acid metabolism as a novel pathogenic factor in gastrointestinal cancers.Molecular and cellular biochemistry · 2025Review
- Immunoinformatics and Evaluation of Peptide Vaccines Derived from Global Hepatitis B Viral HBx and HBc Proteins Critical for Covalently Closed Circular DNA Integrity.Microorganisms · 2023Article
- Editorial: Decoding the genetics of viral evolution.Frontiers in genetics · 2022Article
- AnFrontiers in genetics · 2021Article
- Detection of hyper-conserved regions in hepatitis B virus X gene potentially useful for gene therapy.World journal of gastroenterology · 2018Article
- Hepatitis B virus X protein in liver tumor microenvironment.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2016Review
- Cooperative effects of hepatitis B virus and TNF may play important roles in the activation of metabolic pathways through the activation of NF-κB.International journal of molecular medicine · 2016Article
- Hepatitis B virus, HBx mutants and their role in hepatocellular carcinoma.World journal of gastroenterology · 2014Review
- The Kaposi's sarcoma-associated herpesvirus (KSHV)-induced 5-lipoxygenase-leukotriene B4 cascade plays key roles in KSHV latency, monocyte recruitment, and lipogenesis.Journal of virology · 2014Article
- Single nucleotide polymorphism of SREBF-1 gene associated with an increased risk of endometrial cancer in Chinese women.PloS one · 2014Article
- Hepatitis B virus X protein promotes hepatoma cell proliferation via upregulation of MEKK2.Acta pharmacologica Sinica · 2011Article
- Hepatitis B virus X gene and hepatocarcinogenesis.Journal of gastroenterology · 2011Review
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4 authors at 1 institution in 1 country.
Funding
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Abstract
aimPreviously, we identified a natural mutant of hepatitis B virus X gene (HBx) with a deletion from 382 to 401 base pairs (termed HBxDelta127), which could potently enhance growth of hepatoma cells. In this study, we further investigated the mechanism of increased hepatoma cell growth that was mediated by HBxDelta127.
methodsWe examined the effect of HBxDelta127 on the transcription factor sterol regulatory element-binding protein 1c (SREBP-1c) and fatty acid synthase (FAS) in a model of HepG2-XDelta127 (or H7402-XDelta127) cells, which was stably transfected with HBxDelta127 gene in a human hepatoma HepG2 (or H7402) cell line.
resultsRelative to wild type HBx, HBxDelta127 was able to potently activate SREBP-1c at the levels of promoter activity, mRNA and protein by a luciferase reporter gene assay, RT-PCR and Western blot analysis. Then, using the treatment with MK886, a specific 5-lipoxygenases (5-LOX) inhibitor, (or 5-LOX siRNA) we identified that 5-LOX was responsible for the upregulation of SREBP-1c by luciferase reporter gene assay, RT-PCR and Western blot analysis. Because FAS was a target gene of SREBP-1c, we further showed that HBxDelta127 was able to strongly activate the promoter activity of FAS and upregulated the mRNA expression level of FAS as well, by luciferase reporter gene assay and RT-PCR. In function, flow cytometry analysis revealed that FAS contributed to the growth of hepatoma cells that was mediated by HBxDelta127, using cerulenin (a FAS inhibitor).
conclusionHBxDelta127 promotes hepatoma cell growth through activating SREBP-1c involving 5-LOX.
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