ArticleJournal of bacteriology2010
Spontaneous excision of the Salmonella enterica serovar Enteritidis-specific defective prophage-like element phiSE14.
Article in Journal of bacteriology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 28 citations in OpenAlex.
- Establishment and application of a dual RPA-LFD rapid detection method for Salmonella Pullorum and Salmonella Enteritidis.PloS one · 2025Article
- Genomic and Proteomic Analysis of Six Vi01-like Phages Reveals Wide Host Range and Multiple Tail Spike Proteins.Viruses · 2024Article
- Studies on the Transmission of a Tigecycline Resistance-MediatingMicrobiology spectrum · 2022Article
- The effect of a spontaneous induction prophage, phi458, on biofilm formation and virulence in avian pathogenicFrontiers in microbiology · 2022Article
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- Comparative genomics identifies distinct lineages of S. Enteritidis from Queensland, Australia.PloS one · 2018Article
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- Contribution of Fcγ receptors to human respiratory syncytial virus pathogenesis and the impairment of T-cell activation by dendritic cells.Immunology · 2016Article
- Microarray-based detection of Salmonella enterica serovar Enteritidis genes involved in chicken reproductive tract colonization.Applied and environmental microbiology · 2014Article
- Unconventional circularizable bacterial genetic structures carrying antibiotic resistance determinants.Antimicrobial agents and chemotherapy · 2013Article
- The type VI secretion system encoded in Salmonella pathogenicity island 19 is required for Salmonella enterica serotype Gallinarum survival within infected macrophages.Infection and immunity · 2013Article
- Transposon mutagenesis of Salmonella enterica serovar Enteritidis identifies genes that contribute to invasiveness in human and chicken cells and survival in egg albumen.Infection and immunity · 2012Article
- Streptococcus pneumoniae transposon Tn1545/Tn6003 changes to Tn6002 due to spontaneous excision in circular form of the erm(B)- and aphA3-containing macrolide-aminoglycoside-streptothricin (MAS) element.Antimicrobial agents and chemotherapy · 2012Article
- Characterization of a Streptococcus suis tet(O/W/32/O)-carrying element transferable to major streptococcal pathogens.Antimicrobial agents and chemotherapy · 2012Article
- Characterization of the ELPhiS prophage from Salmonella enterica serovar Enteritidis strain LK5.Applied and environmental microbiology · 2012Article
- Infection of mice by Salmonella enterica serovar Enteritidis involves additional genes that are absent in the genome of serovar Typhimurium.Infection and immunity · 2012Article
- Distribution of classical and nonclassical virulence genes in enterotoxigenic Escherichia coli isolates from Chilean children and tRNA gene screening for putative insertion sites for genomic islands.Journal of clinical microbiology · 2011Article
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
Abstract
Salmonella enterica serovar Enteritidis has emerged as a major health problem worldwide in the last few decades. DNA loci unique to S. Enteritidis can provide markers for detection of this pathogen and may reveal pathogenic mechanisms restricted to this serovar. An in silico comparison of 16 Salmonella genomic sequences revealed the presence of an approximately 12.5-kb genomic island (GEI) specific to the sequenced S. Enteritidis strain NCTC13349. The GEI is inserted at the 5' end of gene ydaO (SEN1377), is flanked by 308-bp imperfect direct repeats (attL and attR), and includes 21 open reading frames (SEN1378 to SEN1398), encoding primarily phage-related proteins. Accordingly, this GEI has been annotated as the defective prophage SE14 in the genome of strain NCTC13349. The genetic structure and location of phiSE14 are conserved in 99 of 103 wild-type strains of S. Enteritidis studied here, including reference strains NCTC13349 and LK5. Notably, an extrachromosomal circular form of phiSE14 was detected in every strain carrying this island. The presence of attP sites in the circular forms detected in NCTC13349 and LK5 was confirmed. In addition, we observed spontaneous loss of a tetRA-tagged version of phiSE14, leaving an empty attB site in the genome of strain NCTC13349. Collectively, these results demonstrate that phiSE14 is an unstable genetic element that undergoes spontaneous excision under standard growth conditions. An internal fragment of phiSE14 designated Sdf I has been used as a serovar-specific genetic marker in PCR-based detection systems and as a tool to determine S. Enteritidis levels in experimental infections. The instability of this region may require a reassessment of its suitability for such applications.
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