ArticleJournal of immunological methods2010
Quantitative measurement of F-actin accumulation at the NK cell immunological synapse.
Article in Journal of immunological methods, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 28 citations in OpenAlex.
- Co-expression of non-signaling CARs enhances EGFRvIII-specific CAR T-cell cytotoxicity against head and neck squamous cell carcinoma.BMC cancer · 2026Article
- Human PLCG2 haploinsufficiency results in a novel natural killer cell immunodeficiency.The Journal of allergy and clinical immunology · 2024Article
- Bioengineering tools for probing intracellular events in T lymphocytes.WIREs mechanisms of disease · 2021Review
- Filamin A Is Required for NK Cell Cytotoxicity at the Expense of Cytokine ProductionFrontiers in immunology · 2021Article
- The Role of the Cytoskeleton in Regulating the Natural Killer Cell Immune Response in Health and Disease: From Signaling Dynamics to Function.Frontiers in cell and developmental biology · 2021Review
- HEM1 deficiency disrupts mTORC2 and F-actin control in inherited immunodysregulatory disease.Science (New York, N.Y.) · 2020Article
- Article
- KIR2DL4-HLAG interaction at human NK cell-oligodendrocyte interfaces regulates IFN-γ-mediated effects.Molecular immunology · 2019Article
- Mutations in PI3K110δ cause impaired natural killer cell function partially rescued by rapamycin treatment.The Journal of allergy and clinical immunology · 2018Article
- Reversible Transgene Expression Reduces Fratricide and Permits 4-1BB Costimulation of CAR T Cells Directed to T-cell Malignancies.Cancer immunology research · 2018Article
- Article
- Quantitative Imaging Approaches to Study the CAR Immunological Synapse.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Review
- RASGRP1 deficiency causes immunodeficiency with impaired cytoskeletal dynamics.Nature immunology · 2016Article
- Tandem CAR T cells targeting HER2 and IL13Rα2 mitigate tumor antigen escape.The Journal of clinical investigation · 2016Article
- Human NK cell development requires CD56-mediated motility and formation of the developmental synapse.Nature communications · 2016Article
- Article
- Defective actin accumulation impairs human natural killer cell function in patients with dedicator of cytokinesis 8 deficiency.The Journal of allergy and clinical immunology · 2013Article
- Human immunodeficiency-causing mutation defines CD16 in spontaneous NK cell cytotoxicity.The Journal of clinical investigation · 2012Article
- IL-2 induces a WAVE2-dependent pathway for actin reorganization that enables WASp-independent human NK cell function.The Journal of clinical investigation · 2011Article
- Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
NK cells are lymphocytes of the innate immune system that can kill target cells after activation signal-induced directional secretion of lytic granule contents. This process depends upon F-actin polymerization at the NK cell immunological synapse (NKIS), which is the dynamic organization of molecules at the interface between the NK cell and target cell. Although F-actin accumulation at the NKIS is easily visualized, the ability to quantify F-actin at the NKIS is required to understand how F-actin reorganization and accumulation enable NK cell function. Here, we demonstrate several novel algorithms for measuring the content of F-actin accumulated at the NKIS with special emphasis upon actin contributed by the NK cell. These algorithms do not rely upon overexpressing fluorescent proteins or preincubating cells with vital fluorescent dyes. Using models of the activating and inhibitory NKIS as well as NK cells expressing fluorescent protein--cell surface receptor fusion proteins, these algorithms were tested and were used to quantitatively demonstrate that F-actin accumulates at the activating, but not at the inhibitory NKIS. With these approaches, we have also established mathematical formulas that should prove valuable in the comprehensive quantitative evaluation of the NKIS and be more broadly applicable in the measurement of the accumulation of any fluorophore at an intercellular junction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.