ArticleBMC plant biology2010
Novel induced mlo mutant alleles in combination with site-directed mutagenesis reveal functionally important domains in the heptahelical barley Mlo protein.
Article in BMC plant biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed.
- Novel CRISPR/Cas9-DerivedInternational journal of molecular sciences · 2026Article
- CRISPR-Cas9-Mediated Knockout ofPlants (Basel, Switzerland) · 2025Article
- Identification of Quantitative Trait Loci Associated with Powdery Mildew Resistance in Spring Barley under Conditions of Southeastern Kazakhstan.Plants (Basel, Switzerland) · 2023Article
- A unique resistance mechanism is associated with RBgh2 barley powdery mildew adult plant resistance.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Article
- Virulence and Genetic Types ofJournal of fungi (Basel, Switzerland) · 2023Article
- CRISPR/Cas9 basedFrontiers in plant science · 2022Article
- Adult resistance genes to barley powdery mildew confer basal penetration resistance associated with broad-spectrum resistance.The plant genome · 2021Article
- The MLO1 powdery mildew susceptibility gene in Lathyrus species: The power of high-density linkage maps in comparative mapping and synteny analysis.The plant genome · 2021Article
- Discovery and Characterization of a Novel TomatoGenes · 2021Article
- CmMLO17 and its partner CmKIC potentially support Alternaria alternata growth in Chrysanthemum morifolium.Horticulture research · 2021Article
- Genome-Wide Characterization of theFrontiers in plant science · 2021Article
- Article
- You Had Me at "MAGIC"!: Four Barley MAGIC Populations Reveal Novel Resistance QTL for Powdery Mildew.Genes · 2020Article
- Physiological Changes in BarleyInternational journal of molecular sciences · 2020Article
- Genome wide characterization revealed MnMLO2 and MnMLO6A as candidate genes involved in powdery mildew susceptibility in mulberry.Molecular biology reports · 2020Article
- Functional characterization of the powdery mildew susceptibility gene SmMLO1 in eggplant (Solanum melongena L.).Transgenic research · 2017Article
- mlo-based powdery mildew resistance in hexaploid bread wheat generated by a non-transgenic TILLING approach.Plant biotechnology journal · 2017Article
- Mining the Cicer arietinum genome for the mildew locus O (Mlo) gene family and comparative evolutionary analysis of the Mlo genes from Medicago truncatula and some other plant species.Journal of plant research · 2017Article
- Frequency of a natural truncated allele ofMolecular breeding : new strategies in plant improvement · 2017Article
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5 authors.
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Abstract
backgroundRecessively inherited natural and induced mutations in the barley Mlo gene confer durable broad-spectrum resistance against the powdery mildew pathogen, Blumeria graminis f.sp. hordei. Mlo codes for a member of a plant-specific family of polytopic integral membrane proteins with unknown biochemical activity. Resistant barley mlo mutant alleles identify amino acid residues that are critical for Mlo function in the context of powdery mildew susceptibility.
resultsWe molecularly analyzed a novel set of induced barley mlo mutants and used site-directed mutagenesis in combination with transient gene expression to unravel novel amino acid residues of functional significance. We integrate these results with previous findings to map functionally important regions of the heptahelical Mlo protein. Our data reveal the second and third cytoplasmic loop as being particularly sensitive to functional impediment by mutational perturbation, suggesting that these regions are critical for the susceptibility-conferring activity of the Mlo protein. In contrast, only mutations in the second but not the third cytoplasmic loop appear to trigger the Endoplasmic Reticulum-localized quality control machinery that ensures the biogenesis of properly folded membrane proteins.
conclusionOur findings identify functionally important regions of the polytopic barley Mlo protein and reveal the differential sensitivity of individual protein domains to cellular quality control.
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