ArticleCytoskeleton (Hoboken, N.J.)2010
Chlamydomonas mutants display reversible deficiencies in flagellar beating and axonemal assembly.
Article in Cytoskeleton (Hoboken, N.J.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 16 citations in OpenAlex.
- Intraflagellar transport speed is sensitive to genetic and mechanical perturbations to flagellar beating.The Journal of cell biology · 2024Article
- Structure of the radial spoke head and insights into its role in mechanoregulation of ciliary beating.Nature structural & molecular biology · 2021Article
- The roles of a flagellar HSP40 ensuring rhythmic beating.Molecular biology of the cell · 2019Article
- General and specific promotion of flagellar assembly by a flagellar nucleoside diphosphate kinase.Molecular biology of the cell · 2017Article
- Radial Spokes-A Snapshot of the Motility Regulation, Assembly, and Evolution of Cilia and Flagella.Cold Spring Harbor perspectives in biology · 2017Review
- Targeted NGS gene panel identifies mutations in RSPH1 causing primary ciliary dyskinesia and a common mechanism for ciliary central pair agenesis due to radial spoke defects.Human molecular genetics · 2014Article
- Anomalies in the motion dynamics of long-flagella mutants of Chlamydomonas reinhardtii.Journal of biological physics · 2013Article
- A flagellar A-kinase anchoring protein with two amphipathic helices forms a structural scaffold in the radial spoke complex.The Journal of cell biology · 2012Article
- The DPY-30 domain and its flanking sequence mediate the assembly and modulation of flagellar radial spoke complexes.Molecular and cellular biology · 2012Article
- The versatile molecular complex component LC8 promotes several distinct steps of flagellar assembly.The Journal of cell biology · 2012Article
- Decay of genes encoding the oomycete flagellar proteome in the downy mildew Hyaloperonospora arabidopsidis.PloS one · 2012Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
Axonemal complexes in flagella are largely prepackaged in the cell body. As such, one mutation often results in the absence of the co-assembled components and permanent motility deficiencies. For example, a Chlamydomonas mutant defective in RSP4 in the radial spoke (RS), which is critical for bend propagation, has paralyzed flagella that also lack the paralogue RSP6 and three additional RS proteins. Intriguingly, recent studies showed that several mutant strains contain a mixed population of swimmers and paralyzed cells despite their identical genetic background. Here we report a cause underlying these variations. Two new mutants lacking RSP6 swim processively and other components appear normally assembled in early log phase indicating that, unlike RSP4, this paralogue is dispensable. However, swimmers cannot maintain the typical helical trajectory and reactivated cell models tend to spin. Interestingly the motile fraction and the spokehead content dwindle during stationary phase. These results suggest that (1) intact RS is critical for maintaining the rhythm of oscillatory beating and thus the helical trajectory; (2) assembly of the axonemal complex with subtle defects is less efficient and the inefficiency is accentuated in compromised conditions, leading to reversible dyskinesia. Consistently, several organisms only possess one RSP4/6 gene. Gene duplication in Chlamydomonas enhances RS assembly to maintain optimal motility in various environments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.