ArticleCytoskeleton (Hoboken, N.J.)2010
Efficient spatiotemporal analysis of the flagellar waveform of Chlamydomonas reinhardtii.
Article in Cytoskeleton (Hoboken, N.J.), 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed.
- Embodied behavioural complexity in a ciliated microorganism.Nature communications · 2026Article
- Islet primary cilia motility controls insulin secretion.Science advances · 2022Article
- Generation of ciliary beating by steady dynein activity: the effects of inter-filament coupling in multi-filament models.Journal of the Royal Society, Interface · 2022Article
- Fluorescence imaging of beta cell primary cilia.Frontiers in endocrinology · 2022Article
- CiliaQ: a simple, open-source software for automated quantification of ciliary morphology and fluorescence in 2D, 3D, and 4D images.The European physical journal. E, Soft matter · 2021Article
- Mutation of CFAP57, a protein required for the asymmetric targeting of a subset of inner dynein arms in Chlamydomonas, causes primary ciliary dyskinesia.PLoS genetics · 2020Article
- Computer-assisted beat-pattern analysis and the flagellar waveforms of bovine spermatozoa.Royal Society open science · 2020Article
- Asymmetries in the cilia ofPhilosophical transactions of the Royal Society of London. Series B, Biological sciences · 2020Review
- How Does Cilium Length Affect Beating?Biophysical journal · 2019Article
- Quantifying Ciliary Dynamics during Assembly Reveals Stepwise Waveform Maturation in Airway Cells.American journal of respiratory cell and molecular biology · 2018Article
- Four-dimensional analysis by high-speed holographic imaging reveals a chiral memory of sperm flagella.PloS one · 2018Article
- Ciliary Motility: Regulation of Axonemal Dynein Motors.Cold Spring Harbor perspectives in biology · 2017Review
- Steady dynein forces induce flutter instability and propagating waves in mathematical models of flagella.Journal of the Royal Society, Interface · 2016Article
- Flexural Rigidity and Shear Stiffness of Flagella Estimated from Induced Bends and Counterbends.Biophysical journal · 2016Article
- Dynein-deficient flagella respond to increased viscosity with contrasting changes in power and recovery strokes.Cytoskeleton (Hoboken, N.J.) · 2015Article
- DRC3 connects the N-DRC to dynein g to regulate flagellar waveform.Molecular biology of the cell · 2015Article
- Analysis of unstable modes distinguishes mathematical models of flagellar motion.Journal of the Royal Society, Interface · 2015Article
- Knockdown of Inner Arm Protein IC138 in Trypanosoma brucei Causes Defective Motility and Flagellar Detachment.PloS one · 2015Article
- Equations of interdoublet separation during flagella motion reveal mechanisms of wave propagation and instability.Biophysical journal · 2014Article
- High-throughput phenotyping of chlamydomonas swimming mutants based on nanoscale video analysis.Biophysical journal · 2014Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The 9 + 2 axoneme is a microtubule-based machine that powers the oscillatory beating of cilia and flagella. Its highly regulated movement is essential for the normal function of many organs; ciliopathies cause congenital defects, chronic respiratory tract infections and infertility. We present an efficient method to obtain a quantitative description of flagellar motion, with high spatial and temporal resolution, from high speed video recording of bright field images. This highly automated technique provides the shape, shear angle, curvature, and bend propagation speeds along the length of the flagellum, with approximately 200 temporal samples per beat. We compared the waveforms of uniflagellated wild-type and ida3 mutant cells, which lack the I1 inner dynein complex. Video images were captured at 350 fps. Rigid-body motion was eliminated by fast Fourier transform (FFT)-based registration, and the Cartesian (x-y) coordinates of points on the flagellum were identified. These x-y "point clouds" were embedded in two data dimensions using Isomap, a nonlinear dimension reduction method, and sorted by phase in the flagellar cycle. A smooth surface was fitted to the sorted point clouds, which provides high-resolution estimates of shear angle and curvature. Wild-type and ida3 cells exhibit large differences in shear amplitude, but similar maximum and minimum curvature values. In ida3 cells, the reverse bend begins earlier and travels more slowly relative to the principal bend, than in wild-type cells. The regulation of flagellar movement must involve I1 dynein in a manner consistent with these results.
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