ArticleActa biomaterialia2010
Patterned transgene expression in multiple-channel bridges after spinal cord injury.
Article in Acta biomaterialia, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 40 citations in OpenAlex.
- Non-viral gene delivery systems for tissue repair and regeneration.Journal of translational medicine · 2018Review
- Bridging the lesion-engineering a permissive substrate for nerve regeneration.Regenerative biomaterials · 2015Article
- Gene delivery strategies to promote spinal cord repair.Biomarker insights · 2015Review
- Biomolecule delivery to engineer the cellular microenvironment for regenerative medicine.Annals of biomedical engineering · 2014Review
- Electrospun nanofibers as versatile interfaces for efficient gene delivery.Journal of biological engineering · 2014Review
- The pharmacology of regenerative medicine.Pharmacological reviews · 2013Review
- Biomaterial Scaffolds for Controlled, Localized Gene Delivery of Regenerative Factors.Advances in wound care · 2013Review
- Hydrogels for lentiviral gene delivery.Expert opinion on drug delivery · 2013Review
- Non-viral gene therapy for spinal cord regeneration.Drug discovery today · 2012Review
- Tissue engineering tools for modulation of the immune response.BioTechniques · 2011Review
- Vascular endothelial growth factor and fibroblast growth factor 2 delivery from spinal cord bridges to enhance angiogenesis following injury.Journal of biomedical materials research. Part A · 2011Article
- Engineering biomaterial systems to enhance viral vector gene delivery.Molecular therapy : the journal of the American Society of Gene Therapy · 2011Review
- Spatial control of gene expression within a scaffold by localized inducer release.Biomaterials · 2011Article
- Emerging links between surface nanotechnology and endocytosis: impact on nonviral gene delivery.Nano today · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 1 institution in 1 country.
Funding
Abstract
Patterning of gene delivery on sub-millimeter length scales within tissue engineering scaffolds is fundamental to recreating the complex architectures of tissues. Surface-mediated delivery of lipoplexes mixed with fibronectin was investigated to pattern vectors within 250 microm channels in poly(lactide-co-glycolide) (PLG) bridges. Initial studies performed in vitro on PLG surfaces indicated that a DNA density of 0.07 microg mm(-2) inside each channel with a weight ratio of DNA to fibronectin of 1:20 maximized the number of transfected cells and the levels of transgene expression. Patterned vectors encoding for nerve growth factor (NGF) resulted in localized neurite extension within the channel. Translation to three-dimensional multiple-channel bridges enabled patterned transfection of different vectors throughout the channels for DNA:fibronectin ratios of 1:4 and multiple DNA depositions, with a large increase of neural cell bodies and neurite extension for delivery of DNA encoding for NGF. In vivo, the immobilization of non-viral vectors within the channels resulted in localized transfection within the pore structure of the bridge immediately around the channels of the bridge containing DNA. This surface immobilization strategy enables patterned gene delivery in vitro and in vivo on length scales of hundreds of microns and may find utility in strategies aimed at regenerating tissues with complex architectures.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.