ArticleBMC genomics2010
High-throughput genome sequencing of two Listeria monocytogenes clinical isolates during a large foodborne outbreak.
Article in BMC genomics, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 141 papers.
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Who cites it
141 citing papers in PubMed.
- Listeria monocytogenes isolated from a human case of invasive listeriosis survived more than 25 years in a blood culture bottle.Acta veterinaria Scandinavica · 2026Article
- Clindamycin susceptibility and virulence characterization ofFrontiers in microbiology · 2026Article
- Significance of Whole-Genome Sequencing for the Traceability of Foodborne Pathogens: During the Processing of Meat and Dairy Products.Foods (Basel, Switzerland) · 2025Review
- Molecular Characterization ofFoods (Basel, Switzerland) · 2024Article
- Is the persistence ofApplied and environmental microbiology · 2024Article
- Whole Genome Sequence Analysis ofMicroorganisms · 2024Article
- Phenotypic and genotypic antimicrobial resistance ofThe Lancet regional health. Europe · 2024Article
- Genetic diversity ofAIMS microbiology · 2024Article
- SARS-CoV-2 lineage assignments using phylogenetic placement/UShER are superior to pangoLEARN machine-learning method.Virus evolution · 2024Article
- Detection of Pathogenic Serogroups and Virulence Genes inInternational journal of microbiology · 2024Article
- Association between the Presence of Resistance Genes and Sanitiser Resistance ofMicroorganisms · 2023Article
- Genomic Characterization ofPathogens (Basel, Switzerland) · 2023Article
- Proksee: in-depth characterization and visualization of bacterial genomes.Nucleic acids research · 2023Article
- Human Listeriosis.Clinical microbiology reviews · 2023Review
- Genomic Islands Identified in Highly ResistantMicroorganisms · 2023Article
- Genomic and pathogenicity islands ofFrontiers in molecular biosciences · 2023Review
- Drug-food Interactions in the Era of Molecular Big Data, Machine Intelligence, and Personalized Health.Recent advances in food, nutrition & agriculture · 2022Review
- Whole-Genome Sequence Comparisons ofMicroorganisms · 2022Article
- Genetic Diversity and Relationships ofMicroorganisms · 2022Article
- Article
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Authors and funding
10 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundA large, multi-province outbreak of listeriosis associated with ready-to-eat meat products contaminated with Listeria monocytogenes serotype 1/2a occurred in Canada in 2008. Subtyping of outbreak-associated isolates using pulsed-field gel electrophoresis (PFGE) revealed two similar but distinct AscI PFGE patterns. High-throughput pyrosequencing of two L. monocytogenes isolates was used to rapidly provide the genome sequence of the primary outbreak strain and to investigate the extent of genetic diversity associated with a change of a single restriction enzyme fragment during PFGE.
resultsThe chromosomes were collinear, but differences included 28 single nucleotide polymorphisms (SNPs) and three indels, including a 33 kbp prophage that accounted for the observed difference in AscI PFGE patterns. The distribution of these traits was assessed within further clinical, environmental and food isolates associated with the outbreak, and this comparison indicated that three distinct, but highly related strains may have been involved in this nationwide outbreak. Notably, these two isolates were found to harbor a 50 kbp putative mobile genomic island encoding translocation and efflux functions that has not been observed in other Listeria genomes.
conclusionsHigh-throughput genome sequencing provided a more detailed real-time assessment of genetic traits characteristic of the outbreak strains than could be achieved with routine subtyping methods. This study confirms that the latest generation of DNA sequencing technologies can be applied during high priority public health events, and laboratories need to prepare for this inevitability and assess how to properly analyze and interpret whole genome sequences in the context of molecular epidemiology.
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