ArticleBMC molecular biology2010
Hfq affects mRNA levels independently of degradation.
Article in BMC molecular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
32 citing papers in PubMed, 57 citations in OpenAlex.
- Identification of RNA processing factor LSM5 as a new adverse biomarker in nasopharyngeal carcinoma.Scientific reports · 2025Article
- Characterization of a small non-coding RNA S612 inEngineering microbiology · 2025Article
- Regulation of Ribosomal Protein Synthesis in Mycobacteria: The Autogenous Control ofInternational journal of molecular sciences · 2021Article
- Differential Chromosome- and Plasmid-Borne Resistance ofInternational journal of molecular sciences · 2021Article
- Transcription complexes as RNA chaperones.Transcription · 2021Article
- Autogenous regulation in vivo of theRNA (New York, N.Y.) · 2020Article
- Crystal structure of an Escherichia coli Hfq Core (residues 2-69)-DNA complex reveals multifunctional nucleic acid binding sites.Nucleic acids research · 2020Article
- The Bacterial Amyloid-Like Hfq PromotesMicroorganisms · 2019Article
- New molecular interactions broaden the functions of the RNA chaperone Hfq.Current genetics · 2019Review
- Identification of protein-protein and ribonucleoprotein complexes containing Hfq.Scientific reports · 2019Article
- Inactivation of the RNA helicase CrhR impacts a specific subset of the transcriptome in the cyanobacteriumRNA biology · 2019Article
- Revised role for Hfq bacterial regulator on DNA topology.Scientific reports · 2018Article
- Compaction and condensation of DNA mediated by the C-terminal domain of Hfq.Nucleic acids research · 2017Article
- The small RNA SraG participates in PNPase homeostasis.RNA (New York, N.Y.) · 2016Article
- Regulation of Ribosomal Protein Operons rplM-rpsI, rpmB-rpmG, and rplU-rpmA at the Transcriptional and Translational Levels.Journal of bacteriology · 2016Article
- The Escherichia Coli Hfq Protein: An Unattended DNA-Transactions Regulator.Frontiers in molecular biosciences · 2016Review
- Regulation of the rplY gene encoding 5S rRNA binding protein L25 in Escherichia coli and related bacteria.RNA (New York, N.Y.) · 2015Article
- Effects of Hfq on the conformation and compaction of DNA.Nucleic acids research · 2015Article
- RNA-binding proteins involved in post-transcriptional regulation in bacteria.Frontiers in microbiology · 2015Review
- Clostridium difficile Hfq can replace Escherichia coli Hfq for most of its function.RNA (New York, N.Y.) · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe bacterial Lsm protein, Hfq, is an RNA chaperone involved in many reactions related to RNA metabolism, such as replication and stability, control of small RNA activity and polyadenylation. Despite this wide spectrum of known functions, the global role of Hfq is almost certainly undervalued; its capacity to bind DNA and to interact with many other proteins are only now beginning to be taken into account.
resultsThe role of Hfq in the maturation and degradation of the rpsO mRNA of E. coli was investigated in vivo. The data revealed a decrease in rpsO mRNA abundance concomitant to an increase in its stability when Hfq is absent. This indicates that the change in mRNA levels in hfq mutants does not result from its modification of RNA stability. Moreover, a series of independent experiments have revealed that the decrease in mRNA level is not a consequence of a reduction of translation efficiency and that Hfq is not directly implicated in translational control of rpsO expression. Reduced steady-state mRNA levels in the absence of Hfq were also shown for rpsT, rpsB and rpsB-tsf, but not for lpp, pnp or tRNA transcripts. The abundance of chimeric transcripts rpsO-lacZ and rpsB-lacZ, whose expression was driven by rpsO and rpsB promoters, respectively, was also lower in the hfq null-mutants, while the beta-galactosidase yield remained about the same as in the parent wild-type strain.
conclusionsThe data obtained suggest that alteration of rpsO, rpsT and rpsB-tsf transcript levels observed under conditions of Hfq deficiency is not caused by the post-transcriptional events, such as mRNA destabilization or changes in translation control, and may rather result from changes in transcriptional activity. So far, how Hfq affects transcription remains unclear. We propose that one of the likely mechanisms of Hfq-mediated modulation of transcription might operate early in the elongation step, when interaction of Hfq with a nascent transcript would help to overcome transcription pauses and to prevent preliminary transcript release.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.