ArticleJournal of the Association for Research in Otolaryngology : JARO2010
Supporting cell division is not required for regeneration of auditory hair cells after ototoxic injury in vitro.
Article in Journal of the Association for Research in Otolaryngology : JARO, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 53 citations in OpenAlex.
- EDNRB2 regulates fate, migration, and maturation of hair cell precursors in regenerating avian auditory epithelium explants.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Evidence of interactions among apoptosis, cell proliferation, and dedifferentiation in the rudiment during whole-organ intestinal regeneration in the sea cucumber.Developmental biology · 2024Article
- N-Acetyl-L-cysteine Affects Ototoxicity Evoked by Amikacin and Furosemide Either Alone or in Combination in a Mouse Model of Hearing Threshold Decrease.International journal of molecular sciences · 2023Article
- Stepwise fate conversion of supporting cells to sensory hair cells in the chick auditory epithelium.iScience · 2023Article
- Comparative assessment of Fgf's diverse roles in inner ear development: A zebrafish perspective.Developmental dynamics : an official publication of the American Association of Anatomists · 2021Review
- Differential regulation of mammalian and avian ATOH1 by E2F1 and its implication for hair cell regeneration in the inner ear.Scientific reports · 2021Article
- LIN28B/Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Initiation of Supporting Cell Activation for Hair Cell Regeneration in the Avian Auditory Epithelium: An Explant Culture Model.Frontiers in cellular neuroscience · 2020Article
- Atoh1 is required in supporting cells for regeneration of vestibular hair cells in adult mice.Hearing research · 2020Article
- Transcriptomic analysis of mouse cochleae suffering from gentamicin damage reveals the signalling pathways involved in hair cell regeneration.Scientific reports · 2019Article
- Multiple supporting cell subtypes are capable of spontaneous hair cell regeneration in the neonatal mouse cochlea.Development (Cambridge, England) · 2019Article
- Study of the Mechanisms by Which Aminoglycoside Damage Is Prevented in Chick Embryonic Hair Cells.Journal of the Association for Research in Otolaryngology : JARO · 2019Article
- Bone morphogenetic protein 4 antagonizes hair cell regeneration in the avian auditory epithelium.Hearing research · 2018Article
- Aminoglycoside Damage and Hair Cell Regeneration in the Chicken Utricle.Journal of the Association for Research in Otolaryngology : JARO · 2018Article
- Development and regeneration of vestibular hair cells in mammals.Seminars in cell & developmental biology · 2017Review
- Inhibition of cell proliferation does not slow down echinoderm neural regeneration.Frontiers in zoology · 2017Article
- Article
- Making sense of Wnt signaling-linking hair cell regeneration to development.Frontiers in cellular neuroscience · 2015Review
- Gap junctional coupling is essential for epithelial repair in the avian cochlea.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2014Article
- Lead roles for supporting actors: critical functions of inner ear supporting cells.Hearing research · 2013Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
In chickens, nonsensory supporting cells divide and regenerate auditory hair cells after injury. Anatomical evidence suggests that supporting cells can also transdifferentiate into hair cells without dividing. In this study, we characterized an organ culture model to study auditory hair cell regeneration, and we used these cultures to test if direct transdifferentiation alone can lead to significant hair cell regeneration. Control cultures (organs from posthatch chickens maintained without streptomycin) showed complete hair cell loss in the proximal (high-frequency) region by 5 days. In contrast, a 2-day treatment with streptomycin induced loss of hair cells from all regions by 3 days. Hair cell regeneration proceeded in culture, with the time course of supporting cell division and hair cell differentiation generally resembling in vivo patterns. The degree of supporting cell division depended upon the presence of streptomycin, the epithelial region, the type of culture media, and serum concentration. On average, 87% of the regenerated hair cells lacked the cell division marker BrdU despite its continuous presence, suggesting that most hair cells were regenerated via direct transdifferentiation. Addition of the DNA polymerase inhibitor aphidicolin to culture media prevented supporting cell division, but numerous hair cells were regenerated nonetheless. These hair cells showed signs of functional maturation, including stereociliary bundles and rapid uptake of FM1-43. These observations demonstrate that direct transdifferentiation is a significant mechanism of hair cell regeneration in the chicken auditory after streptomycin damage in vitro.
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