ArticleAmerican journal of physiology. Cell physiology2010
Mapping the cytoskeletal prestress.
Article in American journal of physiology. Cell physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 80 citations in OpenAlex.
- Cytoskeletal prestress homeostasis is a biological principle that governs living cell structure and function.Mechanobiology in medicine · 2026Review
- A life off the beaten track in biomechanics: Imperfect elasticity, cytoskeletal glassiness, and epithelial unjamming.Biophysics reviews · 2023Review
- Balance of osmotic pressures determines the nuclear-to-cytoplasmic volume ratio of the cell.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- In silico stress fibre content affects peak strain in cytoplasm and nucleus but not in the membrane for uniaxial substrate stretch.Medical & biological engineering & computing · 2021Article
- Cytoskeletal prestress: The cellular hallmark in mechanobiology and mechanomedicine.Cytoskeleton (Hoboken, N.J.) · 2021Review
- Increased stiffness and flow resistance of the inner wall of Schlemm's canal in glaucomatous human eyes.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Nasal Polyposis: More than a Chronic Inflammatory Disorder-A Disease of Mechanical Dysfunction-The São Paulo Position.International archives of otorhinolaryngology · 2019Article
- Probe Sensitivity to Cortical versus Intracellular Cytoskeletal Network Stiffness.Biophysical journal · 2019Article
- Mapping heterogeneity of cellular mechanics by multi-harmonic atomic force microscopy.Nature protocols · 2018Article
- Disease-causing mutation in α-actinin-4 promotes podocyte detachment through maladaptation to periodic stretch.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- An Integrated Cytoskeletal Model of Neurite Outgrowth.Frontiers in cellular neuroscience · 2018Review
- Keloid progression: a stiffness gap hypothesis.International wound journal · 2017Article
- Temporal Variation in Single-Cell Power-Law Rheology Spans the Ensemble Variation of Cell Population.Biophysical journal · 2017Article
- A microfluidic chamber-based approach to map the shear moduli of vascular cells and other soft materials.Scientific reports · 2017Article
- Stiffness of pancreatic cancer cells is associated with increased invasive potential.Integrative biology : quantitative biosciences from nano to macro · 2016Article
- Mapping intracellular mechanics on micropatterned substrates.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Article
- Relationship between cell stiffness and stress fiber amount, assessed by simultaneous atomic force microscopy and live-cell fluorescence imaging.Biomechanics and modeling in mechanobiology · 2016Article
- Human bronchial epithelial cells exposed in vitro to diesel exhaust particles exhibit alterations in cell rheology and cytotoxicity associated with decrease in antioxidant defenses and imbalance in pro- and anti-apoptotic gene expression.Environmental science and pollution research international · 2016Article
- High-throughput screening for modulators of cellular contractile force.Integrative biology : quantitative biosciences from nano to macro · 2015Article
Corrections and comments
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Authors and funding
8 authors at 3 institutions in 3 countries.
Funding
Abstract
Cell mechanical properties on a whole cell basis have been widely studied, whereas local intracellular variations have been less well characterized and are poorly understood. To fill this gap, here we provide detailed intracellular maps of regional cytoskeleton (CSK) stiffness, loss tangent, and rate of structural rearrangements, as well as their relationships to the underlying regional F-actin density and the local cytoskeletal prestress. In the human airway smooth muscle cell, we used micropatterning to minimize geometric variation. We measured the local cell stiffness and loss tangent with optical magnetic twisting cytometry and the local rate of CSK remodeling with spontaneous displacements of a CSK-bound bead. We also measured traction distributions with traction microscopy and cell geometry with atomic force microscopy. On the basis of these experimental observations, we used finite element methods to map for the first time the regional distribution of intracellular prestress. Compared with the cell center or edges, cell corners were systematically stiffer and more fluidlike and supported higher traction forces, and at the same time had slower remodeling dynamics. Local remodeling dynamics had a close inverse relationship with local cell stiffness. The principal finding, however, is that systematic regional variations of CSK stiffness correlated only poorly with regional F-actin density but strongly and linearly with the regional prestress. Taken together, these findings in the intact cell comprise the most comprehensive characterization to date of regional variations of cytoskeletal mechanical properties and their determinants.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.