ArticleJournal of clinical microbiology2010
Development of real-time PCR assays for detection of the Streptococcus milleri group from cystic fibrosis clinical specimens by targeting the cpn60 and 16S rRNA genes.
Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 35 citations in OpenAlex.
- Quantitative PCR-Based Analysis of Bacterial Profiles in Periapical Lesions and Maxillary Sinus in Odontogenic Sinusitis.International journal of molecular sciences · 2026Article
- Intramedullary Spinal Cord Abscess With Brain Abscess Secondary to Empyema: A Report of a Rare Case.Cureus · 2026Article
- Auto-aggregation inmBio · 2025Article
- Review
- Uncommon Streptococcus Constellatus Meningitis Leading to Pulmonary Abscess and Brainstem Infarct in an Immunocompetent Patient.The American journal of case reports · 2024Article
- Laboratory Diagnosis, Antimicrobial Susceptibility And Genuine Clinical Spectrum ofMedical archives (Sarajevo, Bosnia and Herzegovina) · 2022Article
- Facial Palsy as Initial Symptom in Glycine Receptor Antibody Positive Progressive Encephalomyelitis With Rigidity and Myoclonus: A Case Report.Frontiers in neurology · 2022Article
- The Clinical View onFrontiers in microbiology · 2022Review
- Empyema caused by Streptococcus constellatus: a case report and literature review.BMC infectious diseases · 2021Review
- From Normal Flora to Brain Abscesses: A Review ofFrontiers in microbiology · 2020Review
- A New Highly Sensitive and Specific Real-Time PCR Assay Targeting the Malate Dehydrogenase Gene of Kingella kingae and Application to 201 Pediatric Clinical Specimens.Journal of clinical microbiology · 2018Article
- Real-time polymerase chain reaction for microbiological diagnosis of parapneumonic effusions in Canadian children.The Canadian journal of infectious diseases & medical microbiology = Journal canadien des maladies infectieuses et de la microbiologie medicale · 2014Article
- Usefulness of PCR-HRMA in identification of non-fermentative Gram-negative rods recovered from patients suffering from cystic fibrosis or chronic obstructive pulmonary disease.Folia microbiologica · 2014Article
- Development of a panel of seven duplex real-time PCR assays for detecting 13 streptococcal superantigens.Annals of clinical microbiology and antimicrobials · 2013Article
- Direct sampling of cystic fibrosis lungs indicates that DNA-based analyses of upper-airway specimens can misrepresent lung microbiota.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- The Streptococcus milleri population of a cystic fibrosis clinic reveals patient specificity and intraspecies diversity.Journal of clinical microbiology · 2010Article
Corrections and comments
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Authors and funding
6 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cystic fibrosis (CF) is a multiorgan disease, with the majority of mortalities resulting from pulmonary failure due to repeated pulmonary exacerbations. Recently, members of the Streptococcus anginosus group (S. anginosus, S. constellatus, and S. intermedius), herein referred to as the "Streptococcus milleri group" (SMG) have been implicated as important etiological pathogens contributing to pulmonary exacerbations in CF patients. This is partly due to better microbiological detection of the SMG species through the development of a novel specific medium termed "McKay agar." McKay agar demonstrated that SMG has been an underreported respiratory pathogen contributing to lung exacerbations. Our aim was to develop a real-time PCR assay to expedite the detection of SMG within diagnostic samples. The cpn60 gene was chosen as a target, with all three members amplified using a single hybridization probe set. SMG strain analysis showed that speciation based on melting curve analysis allowed for the majority of the S. constellatus (96%), S. intermedius (94%), and S. anginosus (60%) strains to be correctly identified. To increase specificity for S. anginosus, two 16S rRNA real-time PCR assays were developed targeting the 16S rRNA gene. The 16s_SA assay is specific for S. anginosus (100%), while the 16s_SCI assay is specific for S. constellatus and S. intermedius (100%). These assays can detect <10 genome equivalents in pure culture and >10(4) genome equivalents in sputum samples, making this a great tool for assessment of the presence of SMG in complex polymicrobial samples. Novel molecular methods were developed providing detection ability for SMG, an emerging opportunistic pathogen.
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