Evidence map›Paper›PMID 20164273›Full record

ArticleJournal of clinical microbiology2010

Comparison of traditional phenotypic identification methods with partial 5' 16S rRNA gene sequencing for species-level identification of nonfermenting Gram-negative bacilli.

Joann L Cloud, Dag Harmsen, Peter C Iwen, James J Dunn, Gerri Hall, Paul Rocco Lasala, Karen Hoggan, Deborah Wilson, Gail L Woods, Alexander Mellmann

Abstract readComparative StudyEvaluation Study
In one paragraph

Article in Journal of clinical microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.

0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

7 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Article
  5. Revisited distribution of nonfermenting Gram-negative bacilli clinical isolates.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2011
    Article
  6. A practical molecular identification of nonfermenting Gram-negative bacteria from cystic fibrosis.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology]
    Article
  7. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Joann L CloudARUP Institute for Clinical and Experimental Pathology, Salt Lake City, Utah 84108, USA. cloudjl@aruplab.com
Dag Harmsen
Peter C Iwen
James J Dunn
Gerri Hall
Paul Rocco Lasala
Karen Hoggan
Deborah Wilson
Gail L Woods
Alexander Mellmann

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Correct identification of nonfermenting Gram-negative bacilli (NFB) is crucial for patient management. We compared phenotypic identifications of 96 clinical NFB isolates with identifications obtained by 5' 16S rRNA gene sequencing. Sequencing identified 88 isolates (91.7%) with >99% similarity to a sequence from the assigned species; 61.5% of sequencing results were concordant with phenotypic results, indicating the usability of sequencing to identify NFB.

Indexed as

Bacterial Typing TechniquesBacteriological TechniquesDNA, BacterialDNA, RibosomalGram-Negative BacteriaGram-Negative Bacterial InfectionsHumansMolecular Sequence DataRNA, Ribosomal, 16SSensitivity and SpecificitySequence Analysis, DNADNA, BacterialDNA, RibosomalRNA, Ribosomal, 16S

Identifiers

PMID20164273
PMCPMC2849612

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.