ArticleThe Journal of biological chemistry2010
The carboxyl-terminal domain of connexin43 is a negative modulator of neuronal differentiation.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 50 citations in OpenAlex.
- The effects of BMP2 and the mechanisms involved in the invasion and angiogenesis of IDH1 mutant glioma cells.Journal of neuro-oncology · 2024Article
- The Gap Junction Inhibitor Octanol Decreases Proliferation and Increases Glial Differentiation of Postnatal Neural Progenitor Cells.International journal of molecular sciences · 2024Article
- Connexin43 in Musculoskeletal System: New Targets for Development and Disease Progression.Aging and disease · 2022Article
- Extracellular vesicles regulate gap junction-mediated intercellular communication and HIV-1 infection of human neural progenitor cells.Neurobiology of disease · 2021Article
- Brain Disorders and Chemical Pollutants: A Gap Junction Link?Biomolecules · 2020Review
- Connexin43 Region 266-283, via Src Inhibition, Reduces Neural Progenitor Cell Proliferation Promoted by EGF and FGF-2 and Increases Astrocytic Differentiation.International journal of molecular sciences · 2020Article
- RhoA/ROCK Regulates Prion Pathogenesis by Controlling Connexin 43 Activity.International journal of molecular sciences · 2020Article
- Connexins and pannexins: At the junction of neuro-glial homeostasis & disease.Journal of neuroscience research · 2018Review
- Loss of Elp3 Impairs the Acetylation and Distribution of Connexin-43 in the Developing Cerebral Cortex.Frontiers in cellular neuroscience · 2017Article
- The C-terminal domain of connexin43 modulates cartilage structure via chondrocyte phenotypic changes.Oncotarget · 2016Article
- Connexins and pannexins in neuronal development and adult neurogenesis.BMC cell biology · 2016Review
- Targeted Gene Resequencing (Astrochip) to Explore the Tripartite Synapse in Autism-Epilepsy Phenotype with Macrocephaly.Neuromolecular medicine · 2016Article
- Transdifferentiation of periodontal ligament-derived stem cells into retinal ganglion-like cells and its microRNA signature.Scientific reports · 2015Article
- Connexin and pannexin signaling pathways, an architectural blueprint for CNS physiology and pathology?Cellular and molecular life sciences : CMLS · 2015Review
- Intracellular Cleavage of the Cx43 C-Terminal Domain by Matrix-Metalloproteases: A Novel Contributor to Inflammation?Mediators of inflammation · 2015Review
- Astroglial connexin 43 sustains glutamatergic synaptic efficacy.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2014Article
- Internal ribosomal entry site (IRES) activity generates endogenous carboxyl-terminal domains of Cx43 and is responsive to hypoxic conditions.The Journal of biological chemistry · 2014Article
- Internal translation of the connexin 43 transcript.Cell communication and signaling : CCS · 2014Article
- Connexin 35b expression in the spinal cord of Danio rerio embryos and larvae.The Journal of comparative neurology · 2014Article
- Cross-regulation of Connexin43 and β-catenin influences differentiation of human neural progenitor cells.Cell death & disease · 2014Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 3 countries.
Funding
Abstract
Connexin43 (Cx43) is widely expressed in embryonic brain, and its expression becomes restricted mainly to astrocytes as the central nervous system matures. Recent studies have indicated that Cx43 plays important, nonchannel, roles during central nervous system development by affecting neuronal cell migration. Here, we evaluated the effects of Cx43 on neuronal differentiation. For that we used an in vitro model of neural cell development (neurospheres) to evaluate, through immunocytochemistry, electrophysiology, and molecular biology, the degree of neuronal maturation from neurospheres derived from wild-type (WT) and Cx43-null mice. Our results indicate that Cx43 is a negative modulator of neuronal differentiation. The percent neurospheres containing differentiated neurons and the number of cells displaying inward currents were significantly higher in Cx43-null than in WT littermate neurospheres. Knockdown of Cx43 with small interfering RNA increased the number of WT neurospheres generating differentiated neurons. Blockade of gap junctional communication with carbenoxolone did not induce neuronal differentiation in WT neurospheres. Transfection of Cx43-null neurospheres with Cx43 mutants revealed that Cx43 carboxyl terminus prevents neuronal maturation. In agreement with these in vitro data, in situ analysis of embryonic day 16 brains revealed increased beta-III-tubulin expression in germinal zones of Cx43-null compared with that of WT littermates. These results indicate that Cx43, and specifically its carboxyl terminus, is crucial for signaling mechanisms preventing premature neuronal differentiation during embryonic brain development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.