ArticleThe Journal of biological chemistry2010
Interactions among HAMP domain repeats act as an osmosensing molecular switch in group III hybrid histidine kinases from fungi.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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34 citing papers in PubMed, 70 citations in OpenAlex.
- Conformational diversity in poly-HAMP arrays and its implications for signal transduction.Protein science : a publication of the Protein Society · 2026Article
- Hybrid histidine kinases shape the architecture of the high osmolarity glycerol (HOG) pathway: from Saccharomyces cerevisiae to the priority pathogen Aspergillus fumigatus and other filamentous fungi.FEMS microbiology reviews · 2026Review
- The N-terminus of themBio · 2024Article
- Article
- Sensing and Responding to Hypersaline Conditions and the HOG Signal Transduction Pathway in Fungi Isolated from Hypersaline Environments:Journal of fungi (Basel, Switzerland) · 2021Review
- Distinct role of HAMP and HAMP-like linker domains in regulating the activity of Hik1p, a hybrid histidine kinase 3 from Magnaporthe oryzae.Molecular genetics and genomics : MGG · 2021Article
- Osmosensing by WNK Kinases.Molecular biology of the cell · 2021Article
- Non-Target Site Mechanisms of Fungicide Resistance in Crop Pathogens: A Review.Microorganisms · 2021Review
- Tolerance to Oxidative Stress in Budding Yeast by Heterologous Expression of Catalases A and T from Debaryomyces hansenii.Current microbiology · 2020Article
- The apical annuli of Toxoplasma gondii are composed of coiled-coil and signalling proteins embedded in the inner membrane complex sutures.Cellular microbiology · 2020Article
- Two Component Regulatory Systems and Antibiotic Resistance in Gram-Negative Pathogens.International journal of molecular sciences · 2019Review
- Phenylpyrrole fungicides act on triosephosphate isomerase to induce methylglyoxal stress and alter hybrid histidine kinase activity.Scientific reports · 2019Article
- Overexpression of the CORVET complex alleviates the fungicidal effects of fludioxonil on the yeastThe Journal of biological chemistry · 2019Article
- Progressive loss of hybrid histidine kinase genes during the evolution of budding yeasts (Saccharomycotina).Current genetics · 2018Article
- Network of nutrient-sensing pathways and a conserved kinase cascade integrate osmolarity and carbon sensing inProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Fludioxonil Induces Drk1, a Fungal Group III Hybrid Histidine Kinase, To Dephosphorylate Its Downstream Target, Ypd1.Antimicrobial agents and chemotherapy · 2017Article
- Phenylpyrroles: 30 Years, Two Molecules and (Nearly) No Resistance.Frontiers in microbiology · 2016Review
- A fungicide-responsive kinase as a tool for synthetic cell fate regulation.Nucleic acids research · 2015Article
- Stress response signaling and virulence: insights from entomopathogenic fungi.Current genetics · 2015Review
- Are Aquaporins the Missing Transmembrane Osmosensors?The Journal of membrane biology · 2015Review
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3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The members of group III hybrid histidine kinases (HHK) are ubiquitous in fungi. Group III HHK have been implicated to function as osmosensors in the high osmolarity glycerol (HOG) pathway that is essential for fungal survival under high osmolarity stress. Recent literature suggests that group III HHK are also involved in conidia formation, virulence in several filamentous fungi, and are an excellent molecular target for antifungal agents. Thus, group III HHK constitute a very important group of sensor kinases. Structurally, group III HHK are distinct from Sln1p, the osmosensing HHK that regulates the HOG pathway in Saccharomyces cerevisiae. Group III HHK lack any transmembrane domain and typically contain HAMP domain repeats at the N terminus. Until now, it is not clear how group III HHK function as an osmosensor to regulate the HOG pathway. To investigate this, we undertook molecular characterization of DhNIK1, an ortholog from osmotolerant yeast Debaryomyces hansenii. We show here that DhNIK1 could complement sln1 mutation in S. cerevisiae thereby confirming its role as a bona fide osmosensor. We further investigated the role of HAMP domains by deleting them systematically. Our results clearly indicate that the HAMP4 domain is crucial for osmosensing by DhNik1p. Most importantly, we also show that the alternative interaction among the HAMP domains regulates the activity of DhNik1p like an "on-off switch" and thus provides, for the first time, an insight into the molecular mechanism of osmosensing by this group of HHKs.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.