ArticleThe Journal of biological chemistry2010
Reconstitution of a functional Toll-like receptor 5 binding site in Campylobacter jejuni flagellin.
Article in The Journal of biological chemistry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 56 citations in OpenAlex.
- Review
- Co-Delivery of Multiple Toll-Like Receptor Agonists and Avian Influenza Hemagglutinin on Protein Nanoparticles Enhances Vaccine Immunogenicity and Efficacy.Advanced healthcare materials · 2025Article
- FlaG competes with FliS-flagellin complexes for access to FlhA in the flagellar T3SS to controlProceedings of the National Academy of Sciences of the United States of America · 2024Article
- The role of the glycome in symbiotic host-microbe interactions.Glycobiology · 2023Review
- Immunogenic Modification of Ligilactobacillus agilis by Specific Amino Acid Substitution of Flagellin.Applied and environmental microbiology · 2022Article
- Host control and the evolution of cooperation in host microbiomes.Nature communications · 2022Article
- The Exopolysaccharide Produced byMicroorganisms · 2021Article
- The Host Cellular Immune Response to Infection by Campylobacter Spp. and Its Role in Disease.Infection and immunity · 2021Review
- A porcine ligated loop model reveals new insight into the host immune response againstGut microbes · 2020Article
- Avian Pattern Recognition Receptor Sensing and Signaling.Veterinary sciences · 2020Review
- Update on Campylobacter vaccine development.Human vaccines & immunotherapeutics · 2019Review
- Induction of Intestinal Th17 Cells by Flagellins From Segmented Filamentous Bacteria.Frontiers in immunology · 2019Article
- Effect of C-Terminus Modification in Salmonella typhimurium FliC on Protein Purification Efficacy and Bioactivity.Molecular biotechnology · 2019Article
- Review
- Functional expression of TLR5 of different vertebrate species and diversification in intestinal pathogen recognition.Scientific reports · 2018Article
- Flagellin Glycoproteomics of the Periodontitis Associated PathogenMolecular & cellular proteomics : MCP · 2018Article
- Studies on the Use of Flagellin as an Immunostimulant and Vaccine Adjuvant in Fish Aquaculture.Frontiers in immunology · 2018Article
- Immunological properties and protective efficacy of a single mycobacterial antigen displayed on polyhydroxybutyrate beads.Microbial biotechnology · 2017Article
- CBLB502 administration protects gut mucosal tissue in ulcerative colitis by inhibiting inflammation.Annals of translational medicine · 2016Article
- Reptile Toll-like receptor 5 unveils adaptive evolution of bacterial flagellin recognition.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bacterial flagellin is important for intestinal immune homeostasis. Flagellins from most species activate Toll-like receptor 5 (TLR5). The principal bacterial food-borne pathogen Campylobacter jejuni escapes TLR5 recognition, probably due to an alternate flagellin subunit structure. We investigated the molecular basis of TLR5 evasion by aiming to reconstitute TLR5 stimulating activity in live C. jejuni. Both native glycosylated C. jejuni flagellins (FlaA and FlaB) and recombinant proteins purified from Escherichia coli failed to activate NF-kappaB in HEK293 cells expressing TLR5. Introduction of multiple defined regions from Salmonella flagellin into C. jejuni FlaA via a recombinatorial approach revealed three regions critical for the activation of human and mouse TLR5, including a beta-hairpin structure not previously implicated in TLR5 recognition. Surprisingly, this domain was not required for the activation of chicken TLR5, indicating a selective requirement for the beta-hairpin in the recognition of mammalian TLR5. Expression of the active chimeric protein in C. jejuni resulted in secreted glycosylated flagellin that induced a potent TLR5 response. Overall, our results reveal a novel structural requirement for TLR5 recognition of bacterial flagellin and exclude flagellin glycosylation as an additional mechanism of bacterial evasion of the TLR5 response.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.