ArticleNucleic acids research2010
Photomodulating RNA cleavage using photolabile circular antisense oligodeoxynucleotides.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 55 citations in OpenAlex.
- Enzyme-Responsive Cyclic Gapmer Antisense Oligonucleotides via ThymidineBioconjugate chemistry · 2026Article
- Chemically inducible antisense oligonucleotides for cell-specific gene silencing.RSC chemical biology · 2025Article
- Topological Switch-OFF and β-Galactosidase-Triggered Switch-ON of Cyclic Antisense Oligonucleotides via CuAAC for Controlled RNA Cleavage.Bioconjugate chemistry · 2025Article
- Single-Round Circular Aptamer Discovery Using Bioinspired Magnetosome-Like Magnetic Chain Cross-Linked Graphene Oxide.Research (Washington, D.C.) · 2024Article
- Cathepsin B-activatable cyclic antisense oligonucleotides for cell-specific target gene knockdownMolecular therapy. Nucleic acids · 2023Article
- Photochemical modifications for DNA/RNA oligonucleotides.RSC advances · 2022Review
- Translational control of gene function through optically regulated nucleic acids.Chemical Society reviews · 2021Review
- Photoactivatable nanoCRISPR/Cas9 System Based on crRNA Reversibly Immobilized on Carbon Nanoparticles.International journal of molecular sciences · 2021Article
- Conditionally Activated ("Caged") Oligonucleotides.Molecules (Basel, Switzerland) · 2021Review
- Evolution of a highly functional circular DNA aptamer in serum.Nucleic acids research · 2020Article
- Optochemical Control of Biological Processes in Cells and Animals.Angewandte Chemie (International ed. in English) · 2018Review
- Hydrogen peroxide-triggered gene silencing in mammalian cells through boronated antisense oligonucleotides.Chemical science · 2018Article
- Stimuli-responsive oligonucleotides in prodrug-based approaches for gene silencing.Beilstein journal of organic chemistry · 2018Review
- Circular Bivalent Aptamers Enable in Vivo Stability and Recognition.Journal of the American Chemical Society · 2017Article
- Caged oligonucleotides for studying biological systems.Journal of inorganic biochemistry · 2015Review
- Optochemical control of deoxyoligonucleotide function via a nucleobase-caging approach.Accounts of chemical research · 2014Article
- Caged oligonucleotides for bidirectional photomodulation of let-7 miRNA in zebrafish embryos.Bioorganic & medicinal chemistry · 2013Article
- Caged circular antisense oligonucleotides for photomodulation of RNA digestion and gene expression in cells.Nucleic acids research · 2013Article
- Manipulation of gene expression in zebrafish using caged circular morpholino oligomers.Nucleic acids research · 2012Article
- Light-controlled synthetic gene circuits.Current opinion in chemical biology · 2012Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Caged antisense oligodeoxynucleotides (asODNs) are synthesized by linking two ends of linear oligodeoxynucleotides using a photocleavable linker. Two of them (H30 and H40) have hairpin-like structures which show a large difference in thermal stability (Delta T(m) = 17.5 degrees C and 11.6 degrees C) comparing to uncaged ones. The other three (C20, C30 and C40) without stable secondary structures have the middle 20 deoxynucleotides complementary to 40-mer RNA. All caged asODNs have restricted opening which provides control over RNA/asODN interaction. RNase H assay results showed that 40-mer RNA digestion could be photo-modulated 2- to 3-fold upon light-activation with H30, H40, C30 and C40, while with C20, RNA digestion was almost not detectable; however, photo-activation triggered >20-fold increase of RNA digestion. And gel shift assays showed that it needed >0.04 microM H40 and 0.5 microM H30 to completely bind 0.02 microM 40-mer RNA, and for C40 and C30, it needed >0.2 microM and 0.5 microM for 0.02 microM 40-mer RNA binding. However, even 4 microM C20 was not able to fully bind the same concentration of 40-mer RNA. By simple adjustment of ring size of caged asODNs, we could successfully photoregulate their hybridization with mRNA and target RNA hydrolysis by RNase H with light activation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.