ArticleMolecular & cellular proteomics : MCP2010
Dual-color proteomic profiling of complex samples with a microarray of 810 cancer-related antibodies.
Article in Molecular & cellular proteomics : MCP, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed.
- Trial
- Optimizing Positive End-Expiratory Pressure in Asymmetric Acute Lung Injury in a Porcine Model: The Role of Transpulmonary Pressure.International journal of molecular sciences · 2025Article
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- Discovery and systematic assessment of early biomarkers that predict progression to severe COVID-19 disease.Communications medicine · 2023Article
- Proteomic analysis of peritoneal fluid identified COMP and TGFBI as new candidate biomarkers for endometriosis.Scientific reports · 2021Article
- Novel voltammetric tumor necrosis factor-alpha (TNF-α) immunosensor based on gold nanoparticles involved in thiol-functionalized multi-walled carbon nanotubes and bimetallic Ni/Cu-MOFs.Analytical and bioanalytical chemistry · 2021Article
- Three-Color Imaging Enables Simultaneous Screening of Multiple RNA Targets on Small Molecule Microarrays.Current protocols in chemical biology · 2020Article
- Re-Evaluating the Conventional Wisdom about Binding Assays.Trends in biochemical sciences · 2020Review
- Current applications of antibody microarrays.Clinical proteomics · 2018Review
- The Human Plasma Proteome Draft of 2017: Building on the Human Plasma PeptideAtlas from Mass Spectrometry and Complementary Assays.Journal of proteome research · 2017Article
- Diagnostic performance enhancement of pancreatic cancer using proteomic multimarker panel.Oncotarget · 2017Article
- Comparison of the tumor cell secretome and patient sera for an accurate serum-based diagnosis of pancreatic ductal adenocarcinoma.Oncotarget · 2017Article
- Antibody Array Revealed PRL-3 Affects Protein Phosphorylation and Cytokine Secretion.PloS one · 2017Article
- Protein Profiling Gastric Cancer and Neighboring Control Tissues Using High-Content Antibody Microarrays.Microarrays (Basel, Switzerland) · 2016Article
- The Statistical Value of Raw Fluorescence Signal in Luminex xMAP Based Multiplex Immunoassays.Scientific reports · 2016Article
- Contributions of immunoaffinity chromatography to deep proteome profiling of human biofluids.Journal of chromatography. B, Analytical technologies in the biomedical and life sciences · 2016Review
- Technical Advances of the Recombinant Antibody Microarray Technology Platform for Clinical Immunoproteomics.PloS one · 2016Article
- Clinical proteomics: promises, challenges and limitations of affinity arrays.Proteomics. Clinical applications · 2015Review
- Spatiotemporal proteomic analyses during pancreas cancer progression identifies serine/threonine stress kinase 4 (STK4) as a novel candidate biomarker for early stage disease.Molecular & cellular proteomics : MCP · 2014Article
- Article
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13 authors.
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Abstract
Antibody microarrays have the potential to enable comprehensive proteomic analysis of small amounts of sample material. Here, protocols are presented for the production, quality assessment, and reproducible application of antibody microarrays in a two-color mode with an array of 1,800 features, representing 810 antibodies that were directed at 741 cancer-related proteins. In addition to measures of array quality, we implemented indicators for the accuracy and significance of dual-color detection. Dual-color measurements outperform a single-color approach concerning assay reproducibility and discriminative power. In the analysis of serum samples, depletion of high-abundance proteins did not improve technical assay quality. On the contrary, depletion introduced a strong bias in protein representation. In an initial study, we demonstrated the applicability of the protocols to proteins derived from urine samples. We identified differences between urine samples from pancreatic cancer patients and healthy subjects and between sexes. This study demonstrates that biomedically relevant data can be produced. As demonstrated by the thorough quality analysis, the dual-color antibody array approach proved to be competitive with other proteomic techniques and comparable in performance to transcriptional microarray analyses.
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