ArticleBiological psychiatry2010
Flexible Accelerated STOP Tetracycline Operator-knockin (FAST): a versatile and efficient new gene modulating system.
Article in Biological psychiatry, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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Who cites it
64 citing papers in PubMed.
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- KCC2 downregulation after sciatic nerve injury enhances motor function recovery.Scientific reports · 2023Article
- Dermal macrophages set pain sensitivity by modulating the amount of tissue NGF through an SNX25-Nrf2 pathway.Nature immunology · 2023Article
- Downregulation of Bdnf Expression in Adult Mice Causes Body Weight Gain.Neurochemical research · 2022Article
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- Developmental impact of glutamate transporter overexpression on dopaminergic neuron activity and stereotypic behavior.Molecular psychiatry · 2022Article
- The Mlc1 Promoter Directs Müller Cell-specific Gene Expression in the Retina.Translational vision science & technology · 2022Article
- Oligodendrocytic NaNature communications · 2021Article
- Region-Specific and State-Dependent Astrocyte CaThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2021Article
- IL-6 can singlehandedly drive many features of frailty in mice.GeroScience · 2021Article
- TorsinA restoration in a mouse model identifies a critical therapeutic window for DYT1 dystonia.The Journal of clinical investigation · 2021Article
- Astrocytic cAMP modulates memory via synaptic plasticity.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Global knockdown of glutamate decarboxylase 67 elicits emotional abnormality in mice.Molecular brain · 2021Article
- Animal Models for OCD Research.Current topics in behavioral neurosciences · 2021Review
- Neuroligin-3: A Circuit-Specific Synapse Organizer That Shapes Normal Function and Autism Spectrum Disorder-Associated Dysfunction.Frontiers in molecular neuroscience · 2021Review
4 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
We created the Flexible Accelerated STOP Tetracycline Operator (tetO)-knockin (FAST) system, an efficient method for manipulating gene expression in vivo to rapidly screen animal models of disease. A single gene targeting event yields two distinct knockin mice-STOP-tetO and tetO knockin-that permit generation of multiple strains with variable expression patterns: 1) knockout, 2) Cre-mediated rescue, 3) tetracycline-controlled transcriptional activator (tTA)-mediated misexpression, 4) tetracycline-controlled transcriptional activator (tTA)-mediated overexpression, and 5) tetracycline-controlled transcriptional silencer (tTS)-mediated conditional knockout/knockdown. Using the FAST system, multiple gain-of-function and loss-of-function strains can therefore be generated on a time scale not previously achievable. These strains can then be screened for clinically relevant abnormalities. We demonstrate the flexibility and broad applicability of the FAST system by targeting several genes encoding proteins implicated in neuropsychiatric disorders: Mlc1, neuroligin 3, the serotonin 1A receptor, and the serotonin 1B receptor.
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