ArticleBiomedical engineering online2010
Influence of nanofibers on growth and gene expression of human tendon derived fibroblast.
Article in Biomedical engineering online, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers, 1 of them a synthesis that pooled it.
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Who cites it
11 citing papers in PubMed, 1 synthesis or guideline pooled it, 41 citations in OpenAlex.
- Nanofiber Technology for Regenerative Engineering.ACS nano · 2020Pooled it
- Rotator cuff repair and biologic augmentation-what do we know?JSES reviews, reports, and techniques · 2026Review
- Implications of Tissue Engineering for Tendon Repair and Regeneration.Journal of functional biomaterials · 2025Review
- Advances in Stem Cell Therapies for Rotator Cuff Injuries.Frontiers in bioengineering and biotechnology · 2022Review
- Synthetic scaffolds for musculoskeletal tissue engineering: cellular responses to fiber parameters.NPJ Regenerative medicine · 2019Review
- Tendon Fascicle-Inspired Nanofibrous Scaffold of Polylactic acid/Collagen with Enhanced 3D-Structure and Biomechanical Properties.Scientific reports · 2018Article
- Rational design of nanofiber scaffolds for orthopedic tissue repair and regeneration.Nanomedicine (London, England) · 2013Review
- In vitro changes in human tenocyte cultures obtained from proximal biceps tendon: multiple passages result in changes in routine cell markers.Knee surgery, sports traumatology, arthroscopy : official journal of the ESSKA · 2012Article
- Biodegradable synthetic scaffolds for tendon regeneration.Muscles, ligaments and tendons journal · 2012Article
- Biomimetic scaffold design for functional and integrative tendon repair.Journal of shoulder and elbow surgery · 2012Review
- Electrospun PLLA nanofiber scaffolds and their use in combination with BMP-2 for reconstruction of bone defects.PloS one · 2011Article
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Authors and funding
7 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundRotator cuff tears are a common and frequent lesion especially in older patients. The mechanisms of tendon repair are not fully understood. Common therapy options for tendon repair include mini-open or arthroscopic surgery. The use of growth factors in experimental studies is mentioned in the literature. Nanofiber scaffolds, which provide several criteria for the healing process, might be a suitable therapy option for operative treatment. The aim of this study was to explore the effects of nanofiber scaffolds on human tendon derived fibroblasts (TDF's), as well as the gene expression and matrix deposition of these fibroblasts.
methodsNanofibers composed of PLLA and PLLA/Col-I were seeded with human tendon derived fibroblasts and cultivated over a period of 22 days under growth-inductive conditions, and analyzed during the course of culture, with respect to gene expression of different extra cellular matrix components such as collagens, bigylcan and decorin. Furthermore, we measured cell densities and proliferation by using fluorescence microscopy.
resultsPLLA nanofibers possessed a growth inhibitory effect on TDF's. Furthermore, no meaningful influence on the gene expression of collagen I, collagen III and decorin could be observed, while the expression of collagen X increased during the course of cultivation. On the other hand, PLLA/Col-I blend nanofibers had no negative influence on the growth of TDF's. Furthermore, blending PLLA nanofibers with collagen had a positive effect on the gene expression of collagen I, III, X and decorin. Here, gene expression indicated that focal adherence kinases might be involved.
conclusionThis study indicates that the use of nanofibers influence expression of genes associated with the extra cellular matrix formation. The composition of the nanofibers plays a critical role. While PLLA/Col-I blend nanofibers enhance the collagen I and III formation, their expression on PLLA nanofibers was more comparable to controls. However, irrespective of the chemical composition of the fibres, the collagen deposition was altered, an effect which might be associated with a decreased expression of biglycanes.
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