ArticleJournal of cellular physiology2010
Characterization of a stretch-activated potassium channel in chondrocytes.
Article in Journal of cellular physiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 52 citations in OpenAlex.
- Ion Currents Mediated by TRPA1 Channels in Freshly Dissociated Rat Articular Chondrocytes: Biophysical Properties and Regulation by Inflammatory Processes.Pharmaceuticals (Basel, Switzerland) · 2025Article
- Osmotically Sensitive TREK Channels in Rat Articular Chondrocytes: Expression and Functional Role.International journal of molecular sciences · 2024Article
- CaInternational journal of molecular sciences · 2023Review
- The Homeostasis of Cartilage Matrix Remodeling and the Regulation of Volume-Sensitive Ion Channel.Aging and disease · 2022Review
- DeepGANnel: Synthesis of fully annotated single molecule patch-clamp data using generative adversarial networks.PloS one · 2022Article
- Mechanosensory and mechanotransductive processes mediated by ion channels in articular chondrocytes: Potential therapeutic targets for osteoarthritis.Channels (Austin, Tex.) · 2021Review
- Dielectrophoresis as a Tool to Reveal the Potential Role of Ion Channels and Early Electrophysiological Changes in Osteoarthritis.Micromachines · 2021Article
- Physiological Effects of the Electrogenic Current Generated by the NaBioelectricity · 2020Article
- Mechanosensitivity of the BK Channels in Human Glioblastoma Cells: Kinetics and Dynamical Complexity.The Journal of membrane biology · 2018Article
- Detection of TRPV4 channel current-like activity in Fawn Hooded hypertensive (FHH) rat cerebral arterial muscle cells.PloS one · 2017Article
- A New Splice Variant of Large Conductance Ca2+-activated K+ (BK) Channel α Subunit Alters Human Chondrocyte Function.The Journal of biological chemistry · 2016Article
- Activation of a chondrocyte volume-sensitive Cl(-) conductance prior to macroscopic cartilage lesion formation in the rabbit knee anterior cruciate ligament transection osteoarthritis model.Osteoarthritis and cartilage · 2016Article
- Membrane channel gene expression in human costal and articular chondrocytes.Organogenesis · 2016Article
- TRPV4 and K(Ca) ion channels functionally couple as osmosensors in the paraventricular nucleus.British journal of pharmacology · 2015Article
- Physicochemical and biomechanical stimuli in cell-based articular cartilage repair.Current rheumatology reports · 2015Review
- Effects of cyclic equibiaxial mechanical stretch on α-BK and TRPV4 expression in equine chondrocytes.SpringerPlus · 2014Article
- Ion channels and osteoarthritic pain: potential for novel analgesics.Current pain and headache reports · 2013Review
- Fibroblast growth factor 2 drives changes in gene expression following injury to murine cartilage in vitro and in vivo.Arthritis and rheumatism · 2013Article
- Benzamil sensitive ion channels contribute to volume regulation in canine chondrocytes.British journal of pharmacology · 2013Article
- Expression of Transient Receptor Potential Vanilloid (TRPV) channels in different passages of articular chondrocytes.International journal of molecular sciences · 2012Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
Chondrocytes possess the capacity to transduce load-induced mechanical stimuli into electrochemical signals. The aim of this study was to functionally characterize an ion channel activated in response to membrane stretch in isolated primary equine chondrocytes. We used patch-clamp electrophysiology to functionally characterize this channel and immunohistochemistry to examine its distribution in articular cartilage. In cell-attached patch experiments, the application of negative pressures to the patch pipette (in the range of 20-200 mmHg) activated ion channel currents in six of seven patches. The mean activated current was 45.9 +/- 1.1 pA (n = 4) at a membrane potential of 33 mV (cell surface area approximately 240 microm(2)). The mean slope conductance of the principal single channels resolved within the total stretch-activated current was 118 +/- 19 pS (n = 6), and reversed near the theoretical potassium equilibrium potential, E(K+), suggesting it was a high-conductance potassium channel. Activation of these high-conductance potassium channels was inhibited by extracellular TEA (K(d) approx. 900 microM) and iberiotoxin (K(d) approx. 40 nM). This suggests that the current was largely carried by BK-like potassium (MaxiK) channels. To further characterize these BK-like channels, we used inside-out patches of chondrocyte membrane: we found these channels to be activated by elevation in bath calcium concentration. Immunohistochemical staining of equine cartilage samples with polyclonal antibodies to the alpha1- and beta1-subunits of the BK channel revealed positive immunoreactivity for both subunits in superficial zone chondrocytes. These experiments support the hypothesis that functional BK channels are present in chondrocytes and may be involved in mechanotransduction and chemotransduction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.