ArticleCytometry. Part A : the journal of the International Society for Analytical Cytology2010
Association of two clones allows for optimal detection of human FOXP3.
Article in Cytometry. Part A : the journal of the International Society for Analytical Cytology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 30 citations in OpenAlex.
- Increased frequency of CD4 and CD8 regulatory T cells in individuals under 15 years with multibacillary leprosy.PloS one · 2013Trial
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- Lower Functional and Proportional Characteristics of Cord Blood Treg of Male Newborns Compared with Female Newborns.Biomedicines · 2021Article
- The Growing Need to Understand Very Early Onset Inflammatory Bowel Disease.Frontiers in immunology · 2021Review
- Phenotypical characterization of regulatory T cells in humans and rodents.Clinical and experimental immunology · 2016Review
- Monitoring regulatory T cells in clinical samples: consensus on an essential marker set and gating strategy for regulatory T cell analysis by flow cytometry.Cancer immunology, immunotherapy : CII · 2015Article
- Activation of Salmonella Typhi-specific regulatory T cells in typhoid disease in a wild-type S. Typhi challenge model.PLoS pathogens · 2015Article
- Neutrophil recruitment and activation in decidua with intra-amniotic IL-1beta in the preterm rhesus macaque.Biology of reproduction · 2015Article
- Effect of chorioamnionitis on regulatory T cells in moderate/late preterm neonates.Human immunology · 2015Article
- Statins increase the frequency of circulating CD4+ FOXP3+ regulatory T cells in healthy individuals.Journal of immunology research · 2015Article
- Intra-amniotic IL-1β induces fetal inflammation in rhesus monkeys and alters the regulatory T cell/IL-17 balance.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Article
- Impaired function of regulatory T cells in cord blood of children of allergic mothers.Clinical and experimental immunology · 2012Article
- Chronically HIV-1 Infected Patients Exhibit Low Frequencies of CD25+ Regulatory T Cells.The open virology journal · 2012Article
- IL-1α mediated chorioamnionitis induces depletion of FoxP3+ cells and ileal inflammation in the ovine fetal gut.PloS one · 2011Article
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
FOXP3 is a key transcription factor expressed by regulatory T cells (Treg cells). However, differences in staining and analysis protocols have led to conflicting results. Moreover, the transient upregulation of FOXP3 that follows activation in non-Treg cells renders the interpretation of FOXP3 data more difficult in humans than in mice. Human peripheral blood mononuclear cells (PBMCs), isolated CD25(-) or CD25(+)CD4(+) T cells were stained with three different anti-FOXP3 clones (PCH101, 206D, and 259D) alone or in combination, and using different permeabilization methods. FOXP3 expression was evaluated following T cell activation by several pathways. Gating based on a population that did not express FOXP3 (such as CD3(-)CD4(-) T cells) allowed for the optimal characterization of Treg cells. The 206D clone detected a lower percentage of cells than PCH101 or 259D. In contrast, 259D stained a population of activated T cells that PCH101 did not. Staining with two clones together consistently increased the proportion of FOXP3(+) cells. However, it is likely that only the double positive cells are Treg cells, as they expressed the highest CD25 and lowest CD127 levels. Our results emphasize that the choice of staining protocol leads to very different results concerning the frequency of Treg cells in humans. A more consistent identification of these cells will improve the knowledge of their biology, particularly during disease processes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.