ArticleCurrent chemical genomics2008
Improvements in live cell analysis of G protein coupled receptors using second generation BD calcium assay kits.
Article in Current chemical genomics, 2008. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
5 citing papers in PubMed.
- Suppression of SIGMAR1 hinders oral cancer cell growth via modulation of mitochondrial CaMolecular biology reports · 2025Article
- Fluorescence-Based HTS Assays for Ion Channel Modulation in Drug Discovery Pipelines.ChemMedChem · 2024Review
- Quantitative high-throughput assay to measure MC4R-induced intracellular calcium.Journal of molecular endocrinology · 2021Article
- Barcoding T cell calcium response diversity with methods for automated and accurate analysis of cell signals (MAAACS).PLoS computational biology · 2013Article
- A multiplex calcium assay for identification of GPCR agonists and antagonists.Assay and drug development technologies · 2010Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BD Calcium Assay Kits are designed for cell-based calcium mobilization high-throughput screening assays. The kits use a proprietary formulation including a non-fluorescent calcium indicator that becomes activated inside the cell and shows increased fluorescence upon calcium binding. The formulation includes a signal-enhancing reagent to maximize the signal over background in a homogeneous, no-wash assay format, based on a technology developed at BD. We have compared the next generation BD calcium assay kit product family to previous versions of the formulation, and to other commercially available homogeneous calcium assay kits. The improvements have enabled better performance on the cell lines and receptors that we have tested in all plate formats including 1536.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.