ArticlePloS one2010
Human cytomegaloviruses expressing yellow fluorescent fusion proteins--characterization and use in antiviral screening.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Letermovir shows antiviral and neuroprotective effects in differentiating neurons and cerebral organoids mimicking human developing brain.Cellular and molecular life sciences : CMLS · 2026Article
- Latency-associated upregulation of SERBP1 is important for the recruitment of transcriptional repressors to the viral major immediate early promoter of human cytomegalovirus during latent carriage.Frontiers in microbiology · 2022Article
- Valspodar limits human cytomegalovirus infection and dissemination.Antiviral research · 2021Article
- Article
- Human Nasal Turbinate Tissues in Organ Culture as a Model for Human Cytomegalovirus Infection at the Mucosal Entry Site.Journal of virology · 2020Article
- Validation and Characterization of Five Distinct Novel Inhibitors of Human Cytomegalovirus.Journal of medicinal chemistry · 2020Article
- Interferon-Responsive Genes Are Targeted during the Establishment of Human Cytomegalovirus Latency.mBio · 2019Article
- An iPSC-Derived Myeloid Lineage Model of Herpes Virus Latency and Reactivation.Frontiers in microbiology · 2019Article
- The Expression of Human Cytomegalovirus MicroRNA MiR-UL148D during Latent Infection in Primary Myeloid Cells Inhibits Activin A-triggered Secretion of IL-6.Scientific reports · 2016Article
- Transient activation of human cytomegalovirus lytic gene expression during latency allows cytotoxic T cell killing of latently infected cells.Scientific reports · 2016Article
- Development of a high-content screen for the identification of inhibitors directed against the early steps of the cytomegalovirus infectious cycle.Antiviral research · 2015Article
- Glucocorticosteroids trigger reactivation of human cytomegalovirus from latently infected myeloid cells and increase the risk for HCMV infection in D+R+ liver transplant patients.The Journal of general virology · 2015Article
- Latency-associated degradation of the MRP1 drug transporter during latent human cytomegalovirus infection.Science (New York, N.Y.) · 2013Article
- Development of a high-throughput assay to measure the neutralization capability of anti-cytomegalovirus antibodies.Clinical and vaccine immunology : CVI · 2013Article
- Human cytomegalovirus infection of M1 and M2 macrophages triggers inflammation and autologous T-cell proliferation.Journal of virology · 2013Article
- Development of two potential diagnostic monoclonal antibodies against human cytomegalovirus glycoprotein B.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologica · 2012Article
- The cellular protein MCM3AP is required for inhibition of cellular DNA synthesis by the IE86 protein of human cytomegalovirus.PloS one · 2012Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Recombinant viruses labelled with fluorescent proteins are useful tools in molecular virology with multiple applications (e.g., studies on intracellular trafficking, protein localization, or gene activity). We generated by homologous recombination three recombinant cytomegaloviruses carrying the enhanced yellow fluorescent protein (EYFP) fused with the viral proteins IE-2, ppUL32 (pp150), and ppUL83 (pp65). In growth kinetics, the three viruses behaved all like wild type, even at low multiplicity of infection (MOI). The expression of all three fusion proteins was detected, and their respective localizations were the same as for the unmodified proteins in wild-type virus-infected cells. We established the in vivo measurement of fluorescence intensity and used the recombinant viruses to measure inhibition of viral replication by neutralizing antibodies or antiviral substances. The use of these viruses in a pilot screen based on fluorescence intensity and high-content analysis identified cellular kinase inhibitors that block viral replication. In summary, these viruses with individually EYFP-tagged proteins will be useful to study antiviral substances and the dynamics of viral infection in cell culture.
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