ArticlePloS one2010
Role of the gerP operon in germination and outgrowth of Bacillus anthracis spores.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 16 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
16 citing papers in PubMed, 38 citations in OpenAlex.
- Phenotypic analysis of variousApplied and environmental microbiology · 2025Article
- Sporulation at reduced water activity impairs germination kinetics ofApplied and environmental microbiology · 2025Article
- Review
- Genomic identification of germinant receptors and phenotypic response to germinants inCurrent research in microbial sciences · 2025Article
- Updates to Clostridium difficile Spore Germination.Journal of bacteriology · 2018Review
- Proteins Encoded by theApplied and environmental microbiology · 2018Article
- Intestinal calcium and bile salts facilitate germination of Clostridium difficile spores.PLoS pathogens · 2017Article
- Spore Heat Activation Requirements and Germination Responses Correlate with Sequences of Germinant Receptors and with the Presence of a SpecificApplied and environmental microbiology · 2017Article
- A Clostridium difficile-Specific, Gel-Forming Protein Required for Optimal Spore Germination.mBio · 2017Article
- Assessment of Bacterial Communities and Predictive Functional Profiling in Soils Subjected to Short-Term Fumigation-Incubation.Microbial ecology · 2016Article
- Identification of a Novel Lipoprotein Regulator of Clostridium difficile Spore Germination.PLoS pathogens · 2015Article
- Germination of spores of Bacillus species: what we know and do not know.Journal of bacteriology · 2014Review
- Analysis of the effects of a gerP mutation on the germination of spores of Bacillus subtilis.Journal of bacteriology · 2012Article
- Germination and amplification of anthrax spores by soil-dwelling amoebas.Applied and environmental microbiology · 2012Article
- Divergence of the SigB regulon and pathogenesis of the Bacillus cereus sensu lato group.BMC genomics · 2012Article
- Effects of sporulation conditions on the germination and germination protein levels of Bacillus subtilis spores.Applied and environmental microbiology · 2012Article
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
Abstract
Germination of Bacillus anthracis spores occurs when nutrients such as amino acids or purine nucleosides stimulate specific germinant receptors located in the spore inner membrane. The gerP(ABCDEF) operon has been suggested to play a role in facilitating the interaction between germinants and their receptors in spores of Bacillus subtilis and Bacillus cereus. B. anthracis mutants containing deletions in each of the six genes belonging to the orthologue of the gerP(ABCDEF) operon, or deletion of the entire operon, were tested for their ability to germinate. Deletion of the entire gerP operon resulted in a significant delay in germination in response to nutrient germinants. These spores eventually germinated to levels equivalent to wild-type, suggesting that an additional entry point for nutrient germinants may exist. Deletions of each individual gene resulted in a similar phenotype, with the exception of DeltagerPF, which showed no obvious defect. The removal of two additional gerPF-like orthologues was necessary to achieve the germination defect observed for the other mutants. Upon physical removal of the spore coat, the mutant lacking the full gerP operon no longer exhibited a germination defect, suggesting that the GerP proteins play a role in spore coat permeability. Additionally, each of the gerP mutants exhibited a severe defect in calcium-dipicolinic acid (Ca-DPA)-dependent germination, suggesting a role for the GerP proteins in this process. Collectively, these data implicate all GerP proteins in the early stages of spore germination.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.