ArticlePloS one2010
Engineering clostridium strain to accept unmethylated DNA.
Article in PloS one, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 92 citations in OpenAlex.
- Development of a CRISPR-based cytosine base editor for restriction-modification system inactivation to enhance transformation efficiency in Vibrio Sp. dhg.Journal of biological engineering · 2025Article
- Identification of the lipodepsipeptide selethramide encoded in a giant nonribosomal peptide synthetase from aProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Influence of medium modifications (optimization) on high nematicidal activity of the fermentation broth ofFrontiers in bioengineering and biotechnology · 2023Article
- Tuning of Gene Expression inACS synthetic biology · 2022Article
- Article
- Breaking the Restriction Barriers and Applying CRISPRi as a Gene Silencing Tool inMicroorganisms · 2022Article
- Enforcing ATP hydrolysis enhanced anaerobic glycolysis and promoted solvent production in Clostridium acetobutylicum.Microbial cell factories · 2021Article
- Metagenomic Analysis Reveals New Microbiota Related to Fiber Digestion in Pigs.Frontiers in microbiology · 2021Article
- An efficient method for markerless mutant generation by allelic exchange inBiotechnology for biofuels · 2019Article
- Restriction-deficient mutants and marker-less genomic modification for metabolic engineering of the solvent producerBiotechnology for biofuels · 2018Article
- Reexamination of the Physiological Role of PykA in Escherichia coli Revealed that It Negatively Regulates the Intracellular ATP Levels under Anaerobic Conditions.Applied and environmental microbiology · 2017Article
- Extending CRISPR-Cas9 Technology from Genome Editing to Transcriptional Engineering in the Genus Clostridium.Applied and environmental microbiology · 2016Article
- A roadmap for gene system development in Clostridium.Anaerobe · 2016Review
- Elucidating the contributions of multiple aldehyde/alcohol dehydrogenases to butanol and ethanol production in Clostridium acetobutylicum.Scientific reports · 2016Article
- Mutant generation by allelic exchange and genome resequencing of the biobutanol organism Clostridium acetobutylicum ATCC 824.Biotechnology for biofuels · 2016Article
- Gas Fermentation-A Flexible Platform for Commercial Scale Production of Low-Carbon-Fuels and Chemicals from Waste and Renewable Feedstocks.Frontiers in microbiology · 2016Review
- Premethylation of foreign DNA improves integrative transformation efficiency in Synechocystis sp. strain PCC 6803.Applied and environmental microbiology · 2015Article
- Biotechnological applications of mobile group II introns and their reverse transcriptases: gene targeting, RNA-seq, and non-coding RNA analysis.Mobile DNA · 2014Article
- Expansion of the genetic toolkit for metabolic engineering of Clostridium pasteurianum: chromosomal gene disruption of the endogenous CpaAI restriction enzyme.Biotechnology for biofuels · 2014Article
- Developing controllable hypermutable Clostridium cells through manipulating its methyl-directed mismatch repair system.Protein & cell · 2013Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is difficult to genetically manipulate the medically and biotechnologically important genus Clostridium due to the existence of the restriction and modification (RM) systems. We identified and engineered the RM system of a model clostridial species, C. acetobutylicum, with the aim to allow the host to accept the unmethylated DNA efficiently. A gene CAC1502 putatively encoding the type II restriction endonuclease Cac824I was identified from the genome of C. acetobutylicum DSM1731, and disrupted using the ClosTron system based on group II intron insertion. The resulting strain SMB009 lost the type II restriction endonuclease activity, and can be transformed with unmethylated DNA as efficiently as with methylated DNA. The strategy reported here makes it easy to genetically modify the clostridial species using unmethylated DNA, which will help to advance the understanding of the clostridial physiology from the molecular level.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.