ArticlePLoS neglected tropical diseases2010
Transcriptional changes in Schistosoma mansoni during early schistosomula development and in the presence of erythrocytes.
Article in PLoS neglected tropical diseases, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 44 papers.
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Who cites it
44 citing papers in PubMed, 85 citations in OpenAlex.
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- Tetraspanin-enriched microdomains play an important role in pathogenesis in the protozoan parasite Entamoeba histolytica.PLoS pathogens · 2024Article
- Hooked on fish blood: the reliance of a gill parasite on haematophagy.Proceedings. Biological sciences · 2024Article
- Evolution of tetraspanin antigens in the zoonotic Asian blood fluke Schistosoma japonicum.Parasites & vectors · 2023Article
- Identification and characterisation of the haemozoin of Haemonchus contortus.Parasites & vectors · 2023Article
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- Characterization of MicroRNA Cargo of Extracellular Vesicles Isolated From the Plasma ofFrontiers in cellular and infection microbiology · 2022Article
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- T Cell Peptides Derived from Invasive Stages ofJournal of clinical medicine · 2021Article
- Identification of proteins from the secretory/excretory products (SEPs) of the branchiuran ectoparasite Argulus foliaceus (Linnaeus, 1758) reveals unique secreted proteins amongst haematophagous ecdysozoa.Parasites & vectors · 2020Article
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- Schistosoma mansoni schistosomula antigens induce Th1/Pro-inflammatory cytokine responses.Parasite immunology · 2018Article
- Glucose Uptake in the Human Pathogen Schistosoma mansoni Is Regulated Through Akt/Protein Kinase B Signaling.The Journal of infectious diseases · 2018Article
- Treatment of Schistosoma mansoni with miltefosine in vitro enhances serological recognition of defined worm surface antigens.PLoS neglected tropical diseases · 2017Article
- Article
- Molecular characterization of host-parasite cell signalling in Schistosoma mansoni during early development.Scientific reports · 2016Article
- Sex-Biased Transcriptome of Schistosoma mansoni: Host-Parasite Interaction, Genetic Determinants and Epigenetic Regulators Are Associated with Sexual Differentiation.PLoS neglected tropical diseases · 2016Article
- Functional characterisation of Schistosoma japonicum acetylcholinesterase.Parasites & vectors · 2016Article
- Computational deconvolution of gene expression by individual host cellular subsets from microarray analyses of complex, parasite-infected whole tissues.International journal for parasitology · 2016Article
- Lysosome-associated membrane glycoprotein (LAMP)--preliminary study on a hidden antigen target for vaccination against schistosomiasis.Scientific reports · 2015Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundSchistosomes cause more mortality and morbidity than any other human helminth, but control primarily relies on a single drug that kills adult worms. The newly transformed schistosomulum stage is susceptible to the immune response and is a target for vaccine development and rational drug design. METHODOLOGY/PRINCIPAL
findingsTo identify genes which are up-regulated during the maturation of Schistosoma mansoni schistosomula in vitro, we cultured newly transformed parasites for 3 h or 5 days with and without erythrocytes and compared their transcriptional profiles using cDNA microarrays. The most apparent changes were in the up-regulation of genes between 3 h and 5 day schistosomula involved in blood feeding, tegument and cytoskeletal development, cell adhesion, and stress responses. The most highly up-regulated genes included a tegument tetraspanin Sm-tsp-3 (1,600-fold up-regulation), a protein kinase, a novel serine protease and serine protease inhibitor, and intestinal proteases belonging to distinct mechanistic classes. The inclusion of erythrocytes in the culture medium resulted in a general but less pronounced increase in transcriptional activity, with the highest up-regulation of genes involved in iron metabolism, proteolysis, and transport of fatty acids and sugars.
conclusionsWe have identified the genes that are up-regulated during the first 5 days of schistosomula development in vitro. Using a combination of gene silencing techniques and murine protection studies, some of these highly up-regulated transcripts can be targeted for future development of new vaccines and drugs.
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