ArticleCrystal growth & design2009
Microfluidic Generation of Lipidic Mesophases for Membrane Protein Crystallization.
Article in Crystal growth & design, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed.
- Comprehensive microcrystal electron diffraction sample preparation for cryo-EM.Nature protocols · 2025Review
- All polymer microfluidic chips-A fixed target sample delivery workhorse for serial crystallography.Biomicrofluidics · 2023Article
- Demonstration of electron diffraction from membrane protein crystals grown in a lipidic mesophase after lamella preparation by focused ion beam milling at cryogenic temperatures.Journal of applied crystallography · 2020Article
- X-ray transparent microfluidic chips for high-throughput screening and optimization ofBiomicrofluidics · 2017Article
- Graphene-based microfluidics for serial crystallography.Lab on a chip · 2016Article
- Serial Femtosecond Crystallography of Membrane Proteins.Advances in experimental medicine and biology · 2016Article
- An X-ray transparent microfluidic platform for screening of the phase behavior of lipidic mesophases.The Analyst · 2013Article
- A microfluidic approach for protein structure determination at room temperature via on-chip anomalous diffraction.Lab on a chip · 2013Article
- Using macromolecular-crystallography beamline and microfluidic platform for small-angle diffraction studies of lipidic matrices for membrane-protein crystallization.Journal of physics. Conference series · 2013Article
- Fabrication of X-ray compatible microfluidic platforms for protein crystallization.Sensors and actuators. B, Chemical · 2012Article
- Lipidic cubic phase technologies for membrane protein structural studies.Current opinion in structural biology · 2011Review
- Design rules for pumping and metering of highly viscous fluids in microfluidics.Lab on a chip · 2010Article
- Challenges and opportunities for new protein crystallization strategies in structure-based drug design.Expert opinion on drug discovery · 2010Review
- A Plug-Based Microfluidic System for Dispensing Lipidic Cubic Phase (LCP) Material Validated by Crystallizing Membrane Proteins in Lipidic Mesophases.Microfluidics and nanofluidics · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
Abstract
We report on a microfluidic method for the formation of aqueous/lipid mesophases to enable screening of suitable crystallization conditions of membrane proteins from a membrane-like phase in sub-20 nanoliter volumes. This integrated microfluidic chip and the employed mixing strategy address the specific challenges associated with the mixing of fluids of highly different viscosities (here a factor of 30) as well as the non-Newtonian character of the resulting mesophases. The chip requires less than 20 nL of material per condition screened whereas typically on the order of 10 μL or more is needed for a batch preparation in the present screening methods. We validated our approach with the successful crystallization of the membrane protein bacteriorhodopsin.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.