ArticleClinical and translational science2009
Induced pluripotent reprogramming from promiscuous human stemness related factors.
Article in Clinical and translational science, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
28 citing papers in PubMed, 33 citations in OpenAlex.
- Generation of canine induced pluripotent stem cells under feeder-free conditions using Sendai virus vector encoding six canine reprogramming factors.Stem cell reports · 2024Article
- Rat-Induced Pluripotent Stem Cells-Derived Cardiac Myocytes in a Cell Culture Dish.Methods in molecular biology (Clifton, N.J.) · 2022Article
- A novel intranuclear RNA vector system for long-term stem cell modification.Gene therapy · 2016Article
- Regenerative Therapy Prevents Heart Failure Progression in Dyssynchronous Nonischemic Narrow QRS Cardiomyopathy.Journal of the American Heart Association · 2015Article
- Noninvasive 3-dimensional imaging of liver regeneration in a mouse model of hereditary tyrosinemia type 1 using the sodium iodide symporter gene.Liver transplantation : official publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society · 2015Article
- Nuclear reprogramming with a non-integrating human RNA virus.Stem cell research & therapy · 2015Article
- Selection via pluripotency-related transcriptional screen minimizes the influence of somatic origin on iPSC differentiation propensity.Stem cells (Dayton, Ohio) · 2014Article
- Natural cardiogenesis-based template predicts cardiogenic potential of induced pluripotent stem cell lines.Circulation. Cardiovascular genetics · 2013Article
- Disease-causing mitochondrial heteroplasmy segregated within induced pluripotent stem cell clones derived from a patient with MELAS.Stem cells (Dayton, Ohio) · 2013Article
- A comparison of pluripotency and differentiation status of four mesenchymal adult stem cells.Molecular biology reports · 2013Article
- Nuclear reprogramming with c-Myc potentiates glycolytic capacity of derived induced pluripotent stem cells.Journal of cardiovascular translational research · 2013Article
- Intrapatient variations in type 1 diabetes-specific iPS cell differentiation into insulin-producing cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2013Article
- Transgene-free disease-specific induced pluripotent stem cells from patients with type 1 and type 2 diabetes.Stem cells translational medicine · 2012Article
- Energy metabolism plasticity enables stemness programs.Annals of the New York Academy of Sciences · 2012Review
- Article
- Successful disease-specific induced pluripotent stem cell generation from patients with kidney transplantation.Stem cell research & therapy · 2011Article
- Energy metabolism in nuclear reprogramming.Biomarkers in medicine · 2011Review
- Induced pluripotent stem cells from GMP-grade hematopoietic progenitor cells and mononuclear myeloid cells.Stem cell research & therapy · 2011Article
- Variation in mesodermal and hematopoietic potential of adult skin-derived induced pluripotent stem cell lines in mice.Stem cell reviews and reports · 2011Article
- Differentiation MicroRNAs Affect Stemness Status of USSCs.Iranian Red Crescent medical journal · 2011Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
Ectopic expression of pluripotency gene sets provokes nuclear reprogramming in permissive somatic tissue environments generating induced pluripotent stem (iPS) cells. The evolutionary conserved function of stemness orthologs was here tested through interspecies transduction. A spectrum of HIV-based lentiviral vectors was designed, and point mutations in the HIV-1 capsid region identified for efficient infectivity and expanded trans-species tropism. Human pluripotent gene sequences, OCT3/4, SOX2, KLF4 and c-MYC, packaged into engineered lentiviral expression vectors achieved consistent expression in non-human fibroblasts. Despite variation in primary amino-acid sequence between species, introduction of human pluripotent genes produced cell lines with embryonic stem cell-like morphology. Transduced fibroblasts differentiated in vitro into all three germ layers according to gastrulation gene expression profiles, and formed in vivo teratoma with multi-lineage potential. Reprogrammed progeny incorporated into non-human morula to produce blastomeres capable of developing into chimeric embryos with competent organogenesis. This model system establishes a prototypic approach to examine consequences of human stemness factors induced reprogramming in the context of normal embryonic development, exploiting non-human early stage embryos. Thus, ectopic xeno-transduction across species unmasks the promiscuous nature of stemness induction, suggesting evolutionary selection of core processes for somatic tissue reprogramming.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.