ArticleCellular and molecular bioengineering2009
Cyclic Hydraulic Pressure and Fluid Flow Differentially Modulate Cytoskeleton Re-Organization in MC3T3 Osteoblasts.
Article in Cellular and molecular bioengineering, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
31 citing papers in PubMed, 67 citations in OpenAlex.
- Cholinergic regulation of osteocyte mechanobiology: A paradigm for bone adaptation.Science advances · 2025Article
- Mechanosignaling in Osteoporosis: When Cells Feel the Force.International journal of molecular sciences · 2025Review
- Biomechanics of Osseointegration of a Dental Implant in the Mandible Under Shock Wave Therapy: In Silico Study.Materials (Basel, Switzerland) · 2024Article
- Low, but Not High, Pulsating Fluid Shear Stress Affects Matrix Extracellular Phosphoglycoprotein Expression, Mainly via Integrin β Subunits in Pre-Osteoblasts.Current issues in molecular biology · 2024Article
- The emerging role of Piezo1 in the musculoskeletal system and disease.Theranostics · 2024Review
- Derivation, validation, and prediction of loading-induced mineral apposition rates at endocortical and periosteal bone surfaces based on fluid velocity and pore pressure.Bone reports · 2023Article
- The actin cytoskeleton: Morphological changes in pre- and fully developed lung cancer.Biophysics reviews · 2022Review
- Targeted Activation of G-Protein Coupled Receptor-Mediated CaTissue engineering. Part A · 2022Article
- The role of mechanobiology in bone and cartilage model systems in characterizing initiation and progression of osteoarthritis.APL bioengineering · 2022Review
- Characterizing the respiratory-induced mechanical stimulation at the maxillary sinus floor following sinus augmentation by computational fluid dynamics.Frontiers in bioengineering and biotechnology · 2022Article
- Effects of Extracellular Osteoanabolic Agents on the Endogenous Response of Osteoblastic Cells.Cells · 2021Review
- Pulsating fluid flow affects pre-osteoblast behavior and osteogenic differentiation through production of soluble factors.Physiological reports · 2021Article
- Shear Stress Modulates Osteoblast Cell and Nucleus Morphology and Volume.International journal of molecular sciences · 2020Article
- Calcium response in bone cells at different osteogenic stages under unidirectional or oscillatory flow.Biomicrofluidics · 2019Article
- Physiological cyclic hydrostatic pressure induces osteogenic lineage commitment of human bone marrow stem cells: a systematic study.Stem cell research & therapy · 2018Article
- Fluid shear stress impacts ovarian cancer cell viability, subcellular organization, and promotes genomic instability.PloS one · 2018Article
- Microtubules tune mechanotransduction through NOX2 and TRPV4 to decrease sclerostin abundance in osteocytes.Science signaling · 2017Article
- Flowtaxis of osteoblast migration under fluid shear and the effect of RhoA kinase silencing.PloS one · 2017Article
- A Robust Actin Filaments Image Analysis Framework.PLoS computational biology · 2016Article
- Poromicromechanics reveals that physiological bone strains induce osteocyte-stimulating lacunar pressure.Biomechanics and modeling in mechanobiology · 2016Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Mechanical loads are essential towards maintaining bone mass and skeletal integrity. Such loads generate various stimuli at the cellular level, including cyclic hydraulic pressure (CHP) and fluid shear stress (FSS). To gain insight into the anabolic responses of osteoblasts to CHP and FSS, we subjected MC3T3-E1 preosteoblasts to either FSS (12 dynes/cm(2)) or CHP varying from 0 to 68 kPa at 0.5 Hz. As with FSS, CHP produced a significant increase in ATP release over static controls within 5 min of onset. Cell stiffness examined by atomic force microscopy increased after 15 min of either CHP or FSS stimulation, which was attenuated when extracellular ATP was hydrolyzed with apyrase. As previously shown FSS induced polymerization of actins into stress fibers. However, the microtubule network was completely disrupted under FSS. In contrast, CHP appeared to maintain strong microtubule and f-actin networks. The purinergic signaling was found to be involved in the remodeling of f-actin, but not microtubule. Both CHP and FSS applied for 1 hour increased expression of COX-2. These data indicate that, while CHP and FSS produce similar anabolic responses, these stimuli have very different effects on the cytoskeleton remodeling and could contribute to loss of mechanosensitivity with extended loading.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.