ArticleNano today2009
Simultaneous Non-invasive Analysis of DNA Condensation and Stability by Two-step QD-FRET.
Article in Nano today, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed.
- Single-molecule displacement assay reveals strong binding of polyvalent dendrimer ligands to telomeric G-quadruplex.Analytical biochemistry · 2022Article
- DNA-enabled fluorescent-based nanosensors monitoring tumor-related RNA toward advanced cancer diagnosis: A review.Frontiers in bioengineering and biotechnology · 2022Review
- Enhancing FRET biosensing beyond 10 nm with photon avalanche nanoparticles.Nanoscale advances · 2020Article
- Recent Advances in Self-Exciting Photodynamic Therapy.Frontiers in bioengineering and biotechnology · 2020Review
- Oral Nonviral Gene Delivery for Chronic Protein Replacement Therapy.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2018Article
- Polyplexes assembled from self-peptides and regulatory nucleic acids blunt toll-like receptor signaling to combat autoimmunity.Biomaterials · 2017Article
- Quantification of cellular and nuclear uptake rates of polymeric gene delivery nanoparticles and DNA plasmids via flow cytometry.Acta biomaterialia · 2016Article
- Quantum dots in diagnostics and detection: principles and paradigms.The Analyst · 2014Review
- Challenges associated with Penetration of Nanoparticles across Cell and Tissue Barriers: A Review of Current Status and Future Prospects.Nano today · 2014Article
- Recent advances in nanoparticle-based Förster resonance energy transfer for biosensing, molecular imaging and drug release profiling.International journal of molecular sciences · 2012Review
- Understanding nonviral nucleic acid delivery with quantum dot-FRET nanosensors.Nanomedicine (London, England) · 2012Review
- Quantum dot enabled molecular sensing and diagnostics.Theranostics · 2012Article
- Nucleic acid-based nanoengineering: novel structures for biomedical applications.Interface focus · 2011Article
- Uptake and intracellular fate of multifunctional nanoparticles: a comparison between lipoplexes and polyplexes via quantum dot mediated Förster resonance energy transfer.Molecular pharmaceutics · 2011Article
- Tuning physical properties of nanocomplexes through microfluidics-assisted confinement.Nano letters · 2011Article
- Intracellular trafficking and decondensation kinetics of chitosan-pDNA polyplexes.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Article
- Dual-sensitive micellar nanoparticles regulate DNA unpacking and enhance gene-delivery efficiency.Advanced materials (Deerfield Beach, Fla.) · 2010Article
- Balancing protection and release of DNA: tools to address a bottleneck of non-viral gene delivery.Journal of the Royal Society, Interface · 2010Review
- Enzymatic incorporation of multiple dyes for increased sensitivity in QD-FRET sensing for DNA methylation detection.Chembiochem : a European journal of chemical biology · 2010Article
- Quantum dot-based theranostics.Nanoscale · 2010Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Nanoscale vectors comprised of cationic polymers that condense DNA to form nanocomplexes are promising options for gene transfer. The rational design of more efficient nonviral gene carriers will be possible only with better mechanistic understanding of the critical rate-limiting steps, such as nanocomplex unpacking to release DNA and degradation by nucleases. We present a two-step quantum dot fluorescence resonance energy transfer (two-step QD-FRET) approach to simultaneously and non-invasively analyze DNA condensation and stability. Plasmid DNA, double-labeled with QD (525 nm emission) and nucleic acid dyes, were complexed with Cy5-labeled cationic gene carriers. The QD donor drives energy transfer stepwise through the intermediate nucleic acid dye to the final acceptor Cy5. At least three distinct states of DNA condensation and integrity were distinguished in single particle manner and within cells by quantitative ratiometric analysis of energy transfer efficiencies. This novel two-step QD-FRET method allows for more detailed assessment of the onset of DNA release and degradation simultaneously.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.