ArticleNucleic acids research2010
Promotion of exon 6 inclusion in HuD pre-mRNA by Hu protein family members.
Article in Nucleic acids research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
27 citing papers in PubMed, 50 citations in OpenAlex.
- Insights into isoform-specific mineralocorticoid receptor action in the hippocampus.The Journal of endocrinology · 2023Review
- ErbB4 alternative splicing mediates fetal mouse alveolar type II cell differentiation in vitro.Pediatric research · 2022Article
- Regulation of the Alternative Neural Transcriptome by ELAV/Hu RNA Binding Proteins.Frontiers in genetics · 2022Review
- Dynamically expressed single ELAV/Hu orthologue elavl2 of bees is required for learning and memory.Communications biology · 2021Article
- Review
- High-resolution epitope mapping of anti-Hu and anti-Yo autoimmunity by programmable phage display.Brain communications · 2020Article
- Elavl3 is essential for the maintenance of Purkinje neuron axons.Scientific reports · 2018Article
- Interaction of HuDA and PABP at 5'UTR of mouse insulin2 regulates insulin biosynthesis.PloS one · 2018Article
- A-44G transition in SMN2 intron 6 protects patients with spinal muscular atrophy.Human molecular genetics · 2017Article
- Neuronal ELAVL proteins utilize AUF-1 as a co-partner to induce neuron-specific alternative splicing of APP.Scientific reports · 2017Article
- Concentration and Localization of Coexpressed ELAV/Hu Proteins Control Specificity of mRNA Processing.Molecular and cellular biology · 2015Article
- Tyrosine phosphorylation of HuR by JAK3 triggers dissociation and degradation of HuR target mRNAs.Nucleic acids research · 2014Article
- Post-transcriptional regulatory elements and spatiotemporal specification of neocortical stem cells and projection neurons.Neuroscience · 2013Review
- Emerging complexity of the HuD/ELAVl4 gene; implications for neuronal development, function, and dysfunction.RNA (New York, N.Y.) · 2013Review
- RNA protein interaction in neurons.Annual review of neuroscience · 2013Review
- All three RNA recognition motifs and the hinge region of HuC play distinct roles in the regulation of alternative splicing.Nucleic acids research · 2013Article
- A biophysical model for identifying splicing regulatory elements and their interactions.PloS one · 2013Article
- Article
- Alternative splicing of the neurofibromatosis type I pre-mRNA.Bioscience reports · 2012Review
- Neuron-specific ELAV/Hu proteins suppress HuR mRNA during neuronal differentiation by alternative polyadenylation.Nucleic acids research · 2012Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The Hu RNA-binding protein family consists of four members: HuR/A, HuB, HuC and HuD. HuR expression is widespread. The other three neuron-specific Hu proteins play an important role in neuronal differentiation through modulating multiple processes of RNA metabolism. In the splicing events examined previously, Hu proteins promote skipping of the alternative exons. Here, we report the first example where Hu proteins promote inclusion of an alternative exon, exon 6 of the HuD pre-mRNA. Sequence alignment analysis indicates the presence of several conserved AU-rich sequences both upstream and downstream to this alternatively spliced exon. We generated a human HuD exon 6 mini-gene reporter construct that includes these conserved sequences. Hu protein over-expression led to significantly increased exon 6 inclusion from this reporter and endogenous HuD. Studies using truncated and mutant HuD exon 6 reporters demonstrate that two AU-rich sequences located downstream of exon 6 are important. RNAi knockdown of Hu proteins decreased exon 6 inclusion. An in vitro splicing assay indicates that Hu proteins promote HuD exon 6 inclusion directly at the level of splicing. Our studies demonstrate that Hu proteins can function as splicing enhancers and expand the functional role of Hu proteins as splicing regulators.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.