ArticleBMC developmental biology2010
Generation and characterization of a Tet-On (rtTA-M2) transgenic rat.
Article in BMC developmental biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 16 citations in OpenAlex.
- Article
- Utilization of a Strongly InducibleFrontiers in microbiology · 2018Article
- Experimental methods to preserve male fertility and treat male factor infertility.Fertility and sterility · 2016Review
- Inducible, tightly regulated and non-leaky neuronal gene expression in mice.Transgenic research · 2014Article
- A novel TetR-regulating peptide turns off rtTA-mediated activation of gene expression.PloS one · 2014Article
- Airway-specific inducible transgene expression using aerosolized doxycycline.American journal of respiratory cell and molecular biology · 2013Article
- Effects of doxycycline on mesenchymal stem cell chondrogenesis and cartilage repair.Osteoarthritis and cartilage · 2013Article
- Transient, inducible, placenta-specific gene expression in mice.Endocrinology · 2012Article
- Progress on RNAi-based molecular medicines.International journal of nanomedicine · 2012Review
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
backgroundThe tetracycline-inducible gene regulation system is a powerful tool that allows temporal and dose-dependent regulation of target transgene expression in vitro and in vivo. Several tetracycline-inducible transgenic mouse models have been described with ubiquitous or tissue-specific expression of tetracycline-transactivator (tTA), reverse tetracycline-transactivator (rtTA) or Tet repressor (TetR). Here we describe a Tet-On transgenic rat that ubiquitously expresses rtTA-M2 driven by the murine ROSA 26 promoter.
resultsThe homozygous rat line (ROSA-rtTA-M2) generated by lentiviral vector injection, has a single integration site and was derived from the offspring of a genetic mosaic founder with multiple transgene integrations. The rtTA-M2 transgene integrated into an intron of a putative gene on chromosome 2 and does not appear to affect the tissue-specificity or expression of that gene. Fibroblasts from the ROSA-rtTA-M2 rats were transduced with a TetO7/CMV-EGFP lentivirus and exhibited doxycycline dose-dependent expression of the EGFP reporter transgene, in vitro. In addition, doxycycline-inducible EGFP expression was observed, in vivo, when the TetO7/CMV-EGFP lentivirus was injected into testis, kidney and muscle tissues of ROSA-rtTA-M2 rats.
conclusionsThis conditional expression rat model may have application for transgenic overexpression or knockdown studies of gene function in development, disease and gene therapy.
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