ArticleAging2009
The mitochondrial ribosomal protein of the large subunit, Afo1p, determines cellular longevity through mitochondrial back-signaling via TOR1.
Article in Aging, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 50 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
50 citing papers in PubMed, 85 citations in OpenAlex.
- Mitonuclear Communication in Stem Cell Function.Cell proliferation · 2025Review
- Regulators of mitonuclear balance link mitochondrial metabolism to mtDNA expression.Nature cell biology · 2023Article
- Spontaneous Mutation Rates and Spectra of Respiratory-Deficient Yeast.Biomolecules · 2023Article
- Actin Cytoskeleton Regulation by the Yeast NADPH Oxidase Yno1p Impacts Processes Controlled by MAPK Pathways.Antioxidants (Basel, Switzerland) · 2021Article
- Slow Growth and Increased Spontaneous Mutation Frequency in Respiratory DeficientG3 (Bethesda, Md.) · 2020Article
- Insights into the Conserved Regulatory Mechanisms of Human and Yeast Aging.Biomolecules · 2020Review
- Mitochondria orchestrate proteostatic and metabolic stress responses.EMBO reports · 2019Review
- The secret messages between mitochondria and nucleus in muscle cell biology.Archives of biochemistry and biophysics · 2019Review
- Protein synthesis and quality control in aging.Aging · 2018Review
- Mitochondria-cytosol-nucleus crosstalk: learning from Saccharomyces cerevisiae.FEMS yeast research · 2018Review
- Adaptation to metabolic dysfunction during aging: Making the best of a bad situation.Experimental gerontology · 2018Review
- The integrated stress response in budding yeast lifespan extension.Microbial cell (Graz, Austria) · 2017Review
- Article
- Respiratory metabolism and calorie restriction relieve persistent endoplasmic reticulum stress induced by calcium shortage in yeast.Scientific reports · 2016Article
- Mitonuclear communication in homeostasis and stress.Nature reviews. Molecular cell biology · 2016Review
- The rate of metabolism as a factor determining longevity of the Saccharomyces cerevisiae yeast.Age (Dordrecht, Netherlands) · 2016Article
- Dietary restriction, mitochondrial function and aging: from yeast to humans.Biochimica et biophysica acta · 2015Review
- Mechanisms by which different functional states of mitochondria define yeast longevity.International journal of molecular sciences · 2015Review
- Overexpression of Pa_1_10620 encoding a mitochondrial Podospora anserina protein with homology to superoxide dismutases and ribosomal proteins leads to lifespan extension.Current genetics · 2015Article
- Methylation of ribosomal RNA by NSUN5 is a conserved mechanism modulating organismal lifespan.Nature communications · 2015Article
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Authors and funding
19 authors at 5 institutions in 2 countries.
Funding
Abstract
Yeast mother cell-specific aging constitutes a model of replicative aging as it occurs in stem cell populations of higher eukaryotes. Here, we present a new long-lived yeast deletion mutation,afo1 (for aging factor one), that confers a 60% increase in replicative lifespan. AFO1/MRPL25 codes for a protein that is contained in the large subunit of the mitochondrial ribosome. Double mutant experiments indicate that the longevity-increasing action of the afo1 mutation is independent of mitochondrial translation, yet involves the cytoplasmic Tor1p as well as the growth-controlling transcription factor Sfp1p. In their final cell cycle, the long-lived mutant cells do show the phenotypes of yeast apoptosis indicating that the longevity of the mutant is not caused by an inability to undergo programmed cell death. Furthermore, the afo1 mutation displays high resistance against oxidants. Despite the respiratory deficiency the mutant has paradoxical increase in growth rate compared to generic petite mutants. A comparison of the single and double mutant strains for afo1 and fob1 shows that the longevity phenotype of afo1 is independent of the formation of ERCs (ribosomal DNA minicircles). AFO1/MRPL25 function establishes a new connection between mitochondria, metabolism and aging.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.