ArticleAging2009
Sequence-specific processing of telomeric 3' overhangs by the Werner syndrome protein exonuclease activity.
Article in Aging, 2009. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
What it found
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Who cites it
16 citing papers in PubMed, 17 citations in OpenAlex.
- Telomeres and Cancer.Life (Basel, Switzerland) · 2021Review
- Either Rap1 or Cdc13 can protect telomeric single-stranded 3' overhangs from degradation in vitro.Scientific reports · 2019Article
- The Werner Syndrome Helicase Coordinates Sequential Strand Displacement and FEN1-Mediated Flap Cleavage during Polymerase δ Elongation.Molecular and cellular biology · 2017Article
- EXD2 promotes homologous recombination by facilitating DNA end resection.Nature cell biology · 2016Article
- Telomeres and telomerase as therapeutic targets to prevent and treat age-related diseases.F1000Research · 2016Review
- Downregulation of the Werner syndrome protein induces a metabolic shift that compromises redox homeostasis and limits proliferation of cancer cells.Aging cell · 2014Article
- TERRA, hnRNP A1, and DNA-PKcs Interactions at Human Telomeres.Frontiers in oncology · 2013Article
- Early and late steps in telomere overhang processing in normal human cells: the position of the final RNA primer drives telomere shortening.Genes & development · 2012Article
- Cell cycle-regulated association between the Werner syndrome protein and its molecular partners.Cell cycle (Georgetown, Tex.) · 2011Article
- A sequence-dependent exonuclease activity from Tetrahymena thermophila.BMC biochemistry · 2010Article
- Helicase-inactivating mutations as a basis for dominant negative phenotypes.Cell cycle (Georgetown, Tex.) · 2010Review
- Ku80 facilitates chromatin binding of the telomere binding protein, TRF2.Cell cycle (Georgetown, Tex.) · 2010Article
- Processing of human telomeres by the Werner syndrome protein.Cell cycle (Georgetown, Tex.) · 2010Article
- The Werner syndrome protein suppresses telomeric instability caused by chromium (VI) induced DNA replication stress.PloS one · 2010Article
- Mechanistic and biological aspects of helicase action on damaged DNA.Cell cycle (Georgetown, Tex.) · 2010Review
- Altered nuclear functions in progeroid syndromes: a paradigm for aging research.TheScientificWorldJournal · 2009Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Werner syndrome is a premature aging disease caused by loss of function mutations in the Werner syndrome protein (WRN) gene. WRN is a RecQ helicase that in contrast to every other member of this family of proteins possesses an exonuclease activity. The findings that cells lacking WRN activity display accelerated telomere shortening and WRN can be detected at chromosome ends suggest that this protein participates in some aspects of telomere metabolism. In this study we examined the impact of WRN on telomeric substrates with a 3' single-stranded overhang in vitro and show that WRN has sequence-specific exonuclease activity that removes several nucleotides inward with a periodical pattern from the 3' end of the telomeric overhang. This activity is strictly dependent on the presence of telomeric sequences in both the duplex DNA and 3' overhang DNA segment and is strongly inhibited by the telomeric factor POT1 but not TRF2. These data demonstrate that WRN processes telomeric DNA substrates with a 3' single-stranded overhang with high specificity and suggest that this protein could influence the configuration of telomere ends prior to the formation of a protective t-loop structure.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.