ArticleEpigenetics & chromatin2010
Differences in the epigenetic and reprogramming properties of pluripotent and extra-embryonic stem cells implicate chromatin remodelling as an important early event in the developing mouse embryo.
Article in Epigenetics & chromatin, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 45 citations in OpenAlex.
- Asymmetric RBBP7 regulates the first cell fate decision of early mammalian embryos.Cell discovery · 2026Article
- Genomic Reshuffling After Cell Fusion: Catalyst for Cancer Evolution and Progression.Advances in experimental medicine and biology · 2026Review
- Human placental cytotrophoblast epigenome dynamics over gestation and alterations in placental disease.Developmental cell · 2021Article
- The Role of Epigenetics in Placental Development and the Etiology of Preeclampsia.International journal of molecular sciences · 2019Review
- Transition of inner cell mass to embryonic stem cells: mechanisms, facts, and hypotheses.Cellular and molecular life sciences : CMLS · 2019Review
- Unsupervised clustering and epigenetic classification of single cells.Nature communications · 2018Article
- Epigenesis and plasticity of mouse trophoblast stem cells.Cellular and molecular life sciences : CMLS · 2016Review
- Epigenetic inheritance and the missing heritability.Human genomics · 2015Review
- Epigenetic mechanisms of endothelial dysfunction in type 2 diabetes.Clinical epigenetics · 2015Article
- Adaptive mechanisms controlling uterine spiral artery remodeling during the establishment of pregnancy.The International journal of developmental biology · 2014Review
- PcG-mediated higher-order chromatin structures modulate replication programs at the Drosophila BX-C.PLoS genetics · 2013Article
- SATB homeobox proteins regulate trophoblast stem cell renewal and differentiation.The Journal of biological chemistry · 2012Article
- Using heterokaryons to understand pluripotency and reprogramming.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2011Review
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
backgroundDuring early mouse development, two extra-embryonic lineages form alongside the future embryo: the trophectoderm (TE) and the primitive endoderm (PrE). Epigenetic changes known to take place during these early stages include changes in DNA methylation and modified histones, as well as dynamic changes in gene expression.
resultsIn order to understand the role and extent of chromatin-based changes for lineage commitment within the embryo, we examined the epigenetic profiles of mouse embryonic stem (ES), trophectoderm stem (TS) and extra-embryonic endoderm (XEN) stem cell lines that were derived from the inner cell mass (ICM), TE and PrE, respectively. As an initial indicator of the chromatin state, we assessed the replication timing of a cohort of genes in each cell type, based on data that expressed genes and acetylated chromatin domains, generally, replicate early in S-phase, whereas some silent genes, hypoacetylated or condensed chromatin tend to replicate later. We found that many lineage-specific genes replicate early in ES, TS and XEN cells, which was consistent with a broadly 'accessible' chromatin that was reported previously for multiple ES cell lines. Close inspection of these profiles revealed differences between ES, TS and XEN cells that were consistent with their differing lineage affiliations and developmental potential. A comparative analysis of modified histones at the promoters of individual genes showed that in TS and ES cells many lineage-specific regulator genes are co-marked with modifications associated with active (H4ac, H3K4me2, H3K9ac) and repressive (H3K27me3) chromatin. However, in XEN cells several of these genes were marked solely by repressive modifications (such as H3K27me3, H4K20me3). Consistent with TS and XEN having a restricted developmental potential, we show that these cells selectively reprogramme somatic cells to induce the de novo expression of genes associated with extraembryonic differentiation.
conclusionsThese data provide evidence that the diversification of defined embryonic and extra-embryonic lineages is accompanied by chromatin remodelling at specific loci. Stem cell lines from the ICM, TE and PrE can each dominantly reprogramme somatic cells but reset gene expression differently, reflecting their separate lineage identities and increasingly restricted developmental potentials.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.