ArticleJournal of experimental botany2010
Control of starch branching in barley defined through differential RNAi suppression of starch branching enzyme IIa and IIb.
Article in Journal of experimental botany, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed, 183 citations in OpenAlex.
- The SD1 locus affects primary seed dormancy in winter malting barley.The plant genome · 2026Article
- Starch characteristics distinguish malt from non-malt barley and can be modulated viaFrontiers in plant science · 2026Article
- Starch branching enzyme: structure, key functional sites, and regulation of starch characteristics and rice qualities.Plant cell reports · 2025Review
- The miR395b-ABI5 module regulates amylopectin branching and biosynthesis and affects lotus root quality.Plant physiology · 2025Article
- Food as Medicine: Curbing Type-2 Diabetes Prevalence Through Consumption of High Amylose Starchy Foods in Sub-Saharan Africa.Advanced pharmaceutical bulletin · 2025Review
- The transcription factor NnNAC100 positively regulates amylopectin biosynthesis by activating NnSBEII in the rhizome of Nelumbo nucifera Gaertn.Plant cell reports · 2025Article
- Article
- Advances in RNA Interference for Plant Functional Genomics: Unveiling Traits, Mechanisms, and Future Directions.Applied biochemistry and biotechnology · 2024Review
- Mutations in starch BRANCHING ENZYME 2a suppress the traits caused by the loss of ISOAMYLASE1 in barley.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2024Article
- FLOURY ENDOSPERM 6 mutations enhance the sugary phenotype caused by the loss of ISOAMYLASE1 in barley.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023Article
- Tuning heterologous glucan biosynthesis in yeast to understand and exploit plant starch diversity.BMC biology · 2022Article
- Reverse genetic approaches for breeding nutrient-rich and climate-resilient cereal and food legume crops.Heredity · 2022Article
- Suppressed expression of starch branching enzyme 1 and 2 increases resistant starch and amylose content and modifies amylopectin structure in cassava.Plant molecular biology · 2022Article
- Full-Length Transcriptomics Reveals Complex Molecular Mechanism of Salt Tolerance inFrontiers in plant science · 2022Article
- Starch branching enzymes as putative determinants of postharvest quality in horticultural crops.BMC plant biology · 2021Review
- Starch biosynthesis in cereal endosperms: An updated review over the last decade.Plant communications · 2021Review
- Modification of starch composition, structure and properties through editing of TaSBEIIa in both winter and spring wheat varieties by CRISPR/Cas9.Plant biotechnology journal · 2021Article
- Genetic and Environmental Variation in Starch Content, Starch Granule Distribution and Starch Polymer Molecular Characteristics of French Bread Wheat.Foods (Basel, Switzerland) · 2021Article
- Generation and Starch Characterization of Non-Transgenic BEI and BEIIb Double Mutant Rice (Oryza sativa) with Ultra-High Level of Resistant Starch.Rice (New York, N.Y.) · 2021Article
- The Triple Jags of Dietary Fibers in Cereals: How Biotechnology IsFrontiers in plant science · 2021Review
2 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The roles of starch branching enzyme (SBE, EC 2.4.1.18) IIa and SBE IIb in defining the structure of amylose and amylopectin in barley (Hordeum vulgare) endosperm were examined. Barley lines with low expression of SBE IIa or SBE IIb, and with the low expression of both isoforms were generated through RNA-mediated silencing technology. These lines enabled the study of the role of each of these isoforms in determining the amylose content, the distribution of chain lengths, and the frequency of branching in both amylose and amylopectin. In lines where both SBE IIa and SBE IIb expression were reduced by >80%, a high amylose phenotype (>70%) was observed, while a reduction in the expression of either of these isoforms alone had minor impact on amylose content. The structure and properties of the high amylose starch resulting from the concomitant reduction in the expression of both isoforms of SBE II in barley were found to approximate changes seen in amylose extender mutants of maize, which result from lesions eliminating expression of the SBE IIb gene. Amylopectin chain length distribution analysis indicated that both SBE IIa and SBE IIb isoforms play distinct roles in determining the fine structure of amylopectin. A significant reduction in the frequency of branches in amylopectin was noticed only when both SBE IIa and SBE IIb were reduced, whereas there was a significant increase in the branching frequency of amylose when SBE IIb alone was reduced. Functional interactions between SBE isoforms are suggested, and a possible inhibitory role of SBE IIb on other SBE isoforms is discussed.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.