ArticleBMC molecular biology2010
Validation of reference genes for gene expression analysis in chicory (Cichorium intybus) using quantitative real-time PCR.
Article in BMC molecular biology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 116 papers, 1 of them a synthesis that pooled it.
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116 citing papers in PubMed, 1 synthesis or guideline pooled it, 230 citations in OpenAlex.
- Genome-Wide Datasets of Chicories (International journal of molecular sciences · 2023Pooled it
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- Integrated transcriptome and metabolome analysis provides insights into anthocyanin biosynthesis in Cichorium intybus L.BMC plant biology · 2025Article
- Selection and validation of reference genes in alfalfa based on transcriptome sequence data.Scientific reports · 2025Article
- Identification of stable reference genes in Edwardsiella ictaluri for accurate gene expression analysis.PloS one · 2025Article
- Evaluation of Reference Genes for Normalizing RT-qPCR and Analysis of the Expression Patterns ofInternational journal of molecular sciences · 2023Article
- Expression assay of theClinical case reports · 2023Article
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- Stable reference gene selection for quantitative real-time PCR normalization in passion fruit (Passiflora edulis Sims.).Molecular biology reports · 2022Article
- Selection of suitable candidate genes for mRNA expression normalization in bulbil development of Pinellia ternata.Scientific reports · 2022Article
- Entailing the Next-Generation Sequencing and Metabolome for Sustainable Agriculture by Improving Plant Tolerance.International journal of molecular sciences · 2022Review
- Selection of the reference genes for quantitative gene expression by RT-qPCR in the desert plant Stipagrostis pennata.Scientific reports · 2021Article
- Comparative transcriptome analysis provides insights into steviol glycoside synthesis in stevia (Stevia rebaudiana Bertoni) leaves under nitrogen deficiency.Plant cell reports · 2021Article
- Identification and selection of reference genes for gene expression analysis by quantitative real-time PCR in Suaeda glauca's response to salinity.Scientific reports · 2021Article
- Identification and evaluation of antioxidant and reference genes for quantitative real-time PCR in blood ofHeliyon · 2021Article
- Candidate reference genes for quantitative gene expression analysis in Lagerstroemia indica.Molecular biology reports · 2021Article
- Reference genes for quantitative real-time PCR normalization of Cenostigma pyramidale roots under salt stress and mycorrhizal association.Genetics and molecular biology · 2021Article
56 more citing papers are in PubMed but not listed here.
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3 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundQuantitative real-time reverse transcriptase polymerase chain reaction (qRT-PCR) is a sensitive technique for quantifying gene expression levels. One or more appropriate reference genes must be selected to accurately compare mRNA transcripts across different samples and tissues. Thus far, only actin-2 has been used as a reference gene for qRT-PCR in chicory, and a full comparison of several candidate reference genes in chicory has not yet been reported.
resultsSeven candidate reference genes, including nicotinamide adenine dinucleotide dehydrogenase (NADHD), actin (ACT), beta-tubulin (TUB), glyceraldehyde-3-phosphate-dehydrogenase (GADPH), histone H3 (H3), elongation factor 1-alpha (EF) and 18S rRNA (rRNA) were selected to study the expression stability for normalisation of gene expression in chicory. Primer specificity and amplification efficiency were verified for each gene. The expression stability of these genes was analysed across chicory root and leaf tissues using geNorm, NormFinder and BestKeeper software. ACT, EF, and rRNA were the most stable genes as identified by the three different analysis methods. In addition, the use of ACT, EF and GAPDH as reference genes was illustrated by analysing 1-FEHII (FEHII) expression in chicory root and leaf tissues. These analyses revealed the biological variation in FEHII transcript expression among the tissues studied, and between individual plants.
conclusionsgeNorm, NormFinder, and BestKeeper analyses indicated that ACT, EF and rRNA had the highest expression stability across leaf and root tissues, while GAPDH and NADHD showed relatively low expression stability. The results of this study emphasise the importance of validating reference genes for qRT-PCR analysis in chicory. The use of the most stable reference genes such as ACT and EF allows accurate normalisation of gene expression in chicory leaf and root tissues.
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