ArticleEuropean journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology2010
Comparison of microscopy, two xenic culture techniques, conventional and real-time PCR for the detection of Dientamoeba fragilis in clinical stool samples.
Article in European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers, 1 of them a synthesis that pooled it.
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Who cites it
32 citing papers in PubMed, 1 synthesis or guideline pooled it, 87 citations in OpenAlex.
- Does Dientamoeba fragilis cause diarrhea? A systematic review.Parasitology research · 2018Pooled it
- Molecular epidemiology of Dientamoeba fragilis coinfections with Blastocystis sp. and Giardia duodenalis in symptomatic patients from Valencia, Spain.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- Lower prevalence of Blastocystis spp. and Dientamoeba fragilis in ulcerative colitis and association with disease activity.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- Assessment of Prevalence of Dientamoeba fragilis and Its Associated Factors Compared to Those of Giardia lamblia and Blastocystis sp. Among Greek Patients with Suspicion of Intestinal Parasitosis.Advances in experimental medicine and biology · 2026Article
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- Observations on the transmission ofParasitology · 2024Article
- Treatment ofNew microbes and new infections · 2023Article
- Prevalence of Intestinal Parasitoses in a Non-Endemic Setting during a 10-Year Period (2011-2020): A Focus onMicroorganisms · 2022Article
- Selecting a multiplex PCR panel for accurate molecular diagnosis of intestinal protists: a comparative study of AllplexParasite (Paris, France) · 2022Article
- Investigation of neglected protists Blastocystis sp. and Dientamoeba fragilis in immunocompetent and immunodeficient diarrheal patients using both conventional and molecular methods.PLoS neglected tropical diseases · 2021Article
- Dientamoeba fragilis Infection in Patients with Digestive and Non-Digestive Symptoms: A Case-Control Study.The Korean journal of parasitology · 2020Article
- Distribution and relevance of Dientamoeba fragilis and Blastocystis species in gastroenteritis: results from a case-control study.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2020Article
- Paromomycin is superior to metronidazole in Dientamoeba fragilis treatment.International journal for parasitology. Drugs and drug resistance · 2019Article
- Coproscopy and molecular screening for detection of intestinal protozoa.Parasites & vectors · 2017Article
- Dientamoeba fragilis, the Neglected Trichomonad of the Human Bowel.Clinical microbiology reviews · 2016Review
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- Dientamoeba fragilis prevalence coincides with gastrointestinal symptoms in children less than 11 years old in Sweden.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2015Article
- Epidemiology and geographical distribution of enteric protozoan infections in sydney, australia.Journal of public health research · 2014Article
- Molecular testing for clinical diagnosis and epidemiological investigations of intestinal parasitic infections.Clinical microbiology reviews · 2014Review
Corrections and comments
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Authors and funding
6 authors at 5 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Dientamoeba fragilis is a pathogenic protozoan parasite that is notoriously difficult to diagnose. The aim of this study was to determine the gold standard for laboratory detection of D. fragilis. A total of 650 human faecal samples were included in the study. All specimens underwent the following: microscopy using a permanent stain (modified iron-haematoxylin), culture using a modified Boeck and Drbohlav's medium (MBD) and TYGM-9, a conventional polymerase chain reaction (PCR) and a real-time PCR (RT-PCR). The overall prevalence of D. fragilis in the study population was 5.4% (35/650). RT-PCR detected 35 isolates, conventional PCR detected 15 isolates, MBD culture detected 14 isolates, TYGM-9 detected ten isolates, while microscopy detected 12 isolates. RT-PCR detected an additional 15 positive samples compared to the other diagnostic methods, all of which were confirmed by sequencing. When all methods were compared to each other, RT-PCR showed a sensitivity and specificity of 100 and 100%, conventional PCR 42.9 and 100%, MBD culture 40 and 100%, TYGM-9 culture 28.6 and 100%, and microscopy 34.3 and 99%, respectively. These results show that RT-PCR is the diagnostic method of choice for the detection of D. fragilis in clinical samples and, as such, should be considered as the gold standard for diagnosis.
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