ArticleJournal of lipid research2010
Inflammation and insulin resistance induced by trans-10, cis-12 conjugated linoleic acid depend on intracellular calcium levels in primary cultures of human adipocytes.
Article in Journal of lipid research, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 48 citations in OpenAlex.
- Review
- Prostaglandin F2α requires activation of calcium-dependent signalling to trigger inflammation in human myometrium.Frontiers in endocrinology · 2023Article
- miRNAs and Novel Food Compounds Related to the Browning Process.International journal of molecular sciences · 2019Review
- Highly selective isomerization of cottonseed oil into conjugated linoleic acid catalyzed by multiwalled carbon nanotube supported ruthenium.RSC advances · 2019Article
- Role of Fatty Acids in Milk Fat and the Influence of Selected Factors on Their Variability-A Review.Molecules (Basel, Switzerland) · 2018Review
- HADC5 deacetylates MKL1 to dampen TNF-α induced pro-inflammatory gene transcription in macrophages.Oncotarget · 2017Article
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- Effect of BI-1 on insulin resistance through regulation of CYP2E1.Scientific reports · 2016Article
- Low level of trans-10, cis-12 conjugated linoleic acid decreases adiposity and increases browning independent of inflammatory signaling in overweight Sv129 mice.The Journal of nutritional biochemistry · 2015Article
- A review on effects of conjugated linoleic fatty acid (CLA) upon body composition and energetic metabolism.Journal of the International Society of Sports Nutrition · 2015Review
- D609-mediated inhibition of ATP synthesis in neural progenitor cells.Neuroreport · 2014Article
- 10E,12Z-conjugated linoleic acid impairs adipocyte triglyceride storage by enhancing fatty acid oxidation, lipolysis, and mitochondrial reactive oxygen species.Journal of lipid research · 2013Article
- The phospholipase C inhibitor U73122 attenuates trans-10, cis-12 conjugated linoleic acid-mediated inflammatory signaling and insulin resistance in human adipocytes.The Journal of nutrition · 2013Article
- Conjugated linoleic acid reduces adiposity and increases markers of browning and inflammation in white adipose tissue of mice.Journal of lipid research · 2013Article
- Diacylglycerol kinase inhibitor R59022 attenuates conjugated linoleic acid-mediated inflammation in human adipocytes.Journal of lipid research · 2013Article
- Fas activates lipolysis in a Ca2+-CaMKII-dependent manner in 3T3-L1 adipocytes.Journal of lipid research · 2013Article
- Article
- Metabolic diseases and pro- and prebiotics: Mechanistic insights.Nutrition & metabolism · 2012Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
Abstract
We previously demonstrated that trans-10, cis-12 (10,12) conjugated linoleic acid (CLA) induced inflammation and insulin resistance in primary human adipocytes by activating nuclear factor kappaB (NFkappaB) and extracellular signal-related kinase (ERK) signaling. In this study, we demonstrated that the initial increase in intracellular calcium ([Ca2+]i) mediated by 10,12 CLA was attenuated by TMB-8, an inhibitor of calcium release from the endoplasmic reticulum (ER), by BAPTA, an intracellular calcium chelator, and by D609, a phospholipase C (PLC) inhibitor. Moreover, BAPTA, TMB-8, and D609 attenuated 10,12 CLA-mediated production of reactive oxygen species (ROS), activation of ERK1/2 and cJun-NH2-terminal kinase (JNK), and induction of inflammatory genes. 10,12 CLA-mediated binding of NFkappaB to the promoters of interleukin (IL)-8 and cyclooxygenase (COX)-2 and induction of calcium-calmodulin kinase II (CaMKII) beta were attenuated by TMB-8. KN-62, a CaMKII inhibitor, also suppressed 10,12 CLA-mediated ROS production and ERK1/2 and JNK activation. Additionally, KN-62 attenuated 10,12 CLA induction of inflammatory and integrated stress response genes, increase in prostaglandin F2alpha, and suppression of peroxisome proliferator activated receptor gamma protein levels and insulin-stimulated glucose uptake. These data suggest that 10,12 CLA increases inflammation and insulin resistance in human adipocytes, in part by increasing [Ca2+]i levels, particularly calcium from the ER.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.