ArticleApplied and environmental microbiology2010
Sequence-based analysis of secondary-metabolite biosynthesis in marine actinobacteria.
Article in Applied and environmental microbiology, 2010. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
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Who cites it
62 citing papers in PubMed.
- Systematic Assessment of the Impact of Targeted Selection Methods and Environment-Mimicking Culture Conditions on Fungal Natural Product Libraries.Journal of natural products · 2026Article
- Diversity of secondary metabolites from marine Streptomyces with potential anti-tubercular activity: a review.Archives of microbiology · 2025Review
- Cryosphere: a frozen home of microbes and a potential source for drug discovery.Archives of microbiology · 2024Review
- Isolation and Screening of Antibacterial Activity ofArchives of Razi Institute · 2023Article
- Basidiomycete non-reducing polyketide synthases function independently of SAT domains.Fungal biology and biotechnology · 2023Article
- Article
- Insights into the phylogenetic diversity, biological activities, and biosynthetic potential of mangrove rhizosphere Actinobacteria from Hainan Island.Frontiers in microbiology · 2023Article
- The natural product biosynthesis potential of the microbiomes of Earth - Bioprospecting for novel anti-microbial agents in the meta-omics era.Computational and structural biotechnology journal · 2022Review
- Isolation and identification of marine microbial products.Journal, genetic engineering & biotechnology · 2021Review
- Culture and genome-based analysis of four soil Clostridium isolates reveal their potential for antimicrobial production.BMC genomics · 2021Article
- Potential secondary metabolite from Indonesian Actinobacteria (InaCC A758) againstIranian journal of basic medical sciences · 2021Article
- Insights into the Variation in Bioactivities of Closely RelatedMarine drugs · 2021Article
- Review
- Insights into Streptomyces spp. isolated from the rhizospheric soil of Panax notoginseng: isolation, antimicrobial activity and biosynthetic potential for polyketides and non-ribosomal peptides.BMC microbiology · 2020Article
- Antimicrobial biosynthetic potential and diversity of culturable soil actinobacteria from forest ecosystems of Northeast India.Scientific reports · 2020Article
- Comparative Investigation into Formycin A and Pyrazofurin A Biosynthesis Reveals Branch Pathways for the Construction ofApplied and environmental microbiology · 2020Article
- PCR-based Screening Approach: A Rapid Method to Detect the Biosynthetic Potential of Antimicrobials in Actinobacterial Strains.Polish journal of microbiology · 2020Article
- Novel bioactive natural products from bacteria via bioprospecting, genome mining and metabolic engineering.Microbial biotechnology · 2019Review
- Screening and Transcriptional Analysis of Polyketide Synthases and Non-ribosomal Peptide Synthetases in Bacterial Strains From Krubera-Voronja Cave.Frontiers in microbiology · 2019Article
- Seco-Tetracenomycins from the Marine-Derived ActinomyceteMarine drugs · 2018Article
2 more citing papers are in PubMed but not listed here.
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4 authors.
Funding
Abstract
A diverse collection of 60 marine-sediment-derived Actinobacteria representing 52 operational taxonomic units was screened by PCR for genes associated with secondary-metabolite biosynthesis. Three primer sets were employed to specifically target adenylation domains associated with nonribosomal peptide synthetases (NRPSs) and ketosynthase (KS) domains associated with type I modular, iterative, hybrid, and enediyne polyketide synthases (PKSs). In total, two-thirds of the strains yielded a sequence-verified PCR product for at least one of these biosynthetic types. Genes associated with enediyne biosynthesis were detected in only two genera, while 88% of the ketosynthase sequences shared greatest homology with modular PKSs. Positive strains included representatives of families not traditionally associated with secondary-metabolite production, including the Corynebacteriaceae, Gordoniaceae, Intrasporangiaceae, and Micrococcaceae. In four of five cases where phylogenetic analyses of KS sequences revealed close evolutionary relationships to genes associated with experimentally characterized biosynthetic pathways, secondary-metabolite production was accurately predicted. Sequence clustering patterns were used to provide an estimate of PKS pathway diversity and to assess the biosynthetic richness of individual strains. The detection of highly similar KS sequences in distantly related strains provided evidence of horizontal gene transfer, while control experiments designed to amplify KS sequences from Salinispora arenicola strain CNS-205, for which a genome sequence is available, led to the detection of 70% of the targeted PKS pathways. The results provide a bioinformatic assessment of secondary-metabolite biosynthetic potential that can be applied in the absence of fully assembled pathways or genome sequences. The rapid identification of strains that possess the greatest potential to produce new secondary metabolites along with those that produce known compounds can be used to improve the process of natural-product discovery by providing a method to prioritize strains for fermentation studies and chemical analysis.
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