ArticleBiophysical journal1991
Is swelling of the secretory granule matrix the force that dilates the exocytotic fusion pore?
Article in Biophysical journal, 1991. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 32 papers.
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Who cites it
32 citing papers in PubMed, 88 citations in OpenAlex.
- Article
- Toward a unified picture of the exocytotic fusion pore.FEBS letters · 2018Review
- Neuronal Porosome Complex: Secretory Machinery at the Nerve Terminal.Discoveries (Craiova, Romania) · 2017Review
- Annexin A2-dependent actin bundling promotes secretory granule docking to the plasma membrane and exocytosis.The Journal of cell biology · 2015Article
- 'Porosome' discovered nearly 20 years ago provides molecular insights into the kiss-and-run mechanism of cell secretion.Journal of cellular and molecular medicine · 2015Review
- Protein mobility within secretory granules.Biophysical journal · 2014Article
- Proteome of the porosome complex in human airway epithelia: interaction with the cystic fibrosis transmembrane conductance regulator (CFTR).Journal of proteomics · 2014Article
- Post-fusion structural changes and their roles in exocytosis and endocytosis of dense-core vesicles.Nature communications · 2014Article
- Electroanalytical eavesdropping on single cell communication.Analytical chemistry · 2011Review
- F-actin and myosin II accelerate catecholamine release from chromaffin granules.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- The force-driven conformations of heparin studied with single molecule force microscopy.Biophysical journal · 2003Article
- Purification and identification of secernin, a novel cytosolic protein that regulates exocytosis in mast cells.Molecular biology of the cell · 2002Article
- Aquaporin 1 regulates GTP-induced rapid gating of water in secretory vesicles.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Dynamics of fusion pores connecting membranes of different tensions.Biophysical journal · 2000Article
- Regulation of exocytotic fusion by cell inflation.Biophysical journal · 1998Article
- Gi regulation of secretory vesicle swelling examined by atomic force microscopy.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Secretory vesicle pools and rate and kinetics of single vesicle exocytosis in neurosecretory cells.Neurochemical research · 1997Review
- Temporally resolved, independent stages of individual exocytotic secretion events.Biophysical journal · 1996Article
- Membrane skeleton restraint of surface shape change during fusion of erythrocyte membranes: evidence from use of osmotic and dielectrophoretic microforces as probes.Biophysical journal · 1995Article
- Membrane mechanics can account for fusion pore dilation in stages.Biophysical journal · 1995Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
The swelling of the secretory granule matrix which follows fusion has been proposed as the driving force for the rapid expansion of the fusion pore necessary for exocytosis. To test this hypothesis, we have combined simultaneous measurements of secretory granule swelling using videomicroscopy with patch clamp measurements of the time course of the exocytotic fusion pore in mast cells from the beige mouse. We show that isotonic acidic histamine solutions are able to inhibit swelling of the secretory granule matrix both in purified secretory granules lysed by electroporation and in intact cells stimulated to exocytose by guanine nucleotides. In contrast to the inhibitory effects on granule swelling, the rate of expansion of the exocytotic fusion pore is unaffected. Therefore, as the rate of granule swelling was more than 20 times slower under these conditions, swelling of the secretory granule matrix due to water entry through the fusion pore cannot be the force responsible for the characteristic rapid expansion of the exocytotic fusion pore. We suggest that tension in the secretory granule membrane, which has recently been demonstrated in fused secretory granules, might be the force that drives the irreversible expansion of the fusion pore.
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